A study of genetic diversity and genome organization of Brassica napus using EST (expressed sequence tags) of Arabidopsis and SSR (simple sequence repeat) markers of B. napus /

• Main Author: Pollock, Stephanie.
• Other Authors: Cheung, Wing Y. (advisor)
• Format: Others
• Language: en
• Published: McGill University 2001
• Subjects: Rape (Plant) > Variation.; Rape (Plant) > Genome mapping.; Arabidopsis thaliana > Molecular genetics.
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33023

Arabidopsis expressed sequence tags (ESTs) and microsatellites of Brassica napus have been developed and used as PCR-based markers for both mapping and genetic diversity studies in B. napus . Out of 300 random Arabidopsis ESTs screened, 43 markers were mapped onto a genetic map of B. napus and then used in a diversity study involving 48 B. napus cultivars. A second set of EST markers were developed from chromosome 1 of Arabidopsis and used in genetic mapping studies of B. napus. From 192 primer pairs developed, 50 markers were added onto the B. napus reference map. Microsatellite markers were developed using a "GA" enriched genomic library from B. napus. From 152 designed primer pairs, 23 markers were added onto the B. napus reference map. Microsatellite markers were also used in genetic diversity studies of B. napus, where, from the 152 primer pairs, 40 revealed polymorphism between the 48 B. napus cultivars.