Nuclear regulation of mitochondrial gene expression in Brassica napus

Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act...

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Main Author: Hamel, Nancy.
Other Authors: Brown, Gregory G. (advisor)
Format: Others
Language:en
Published: McGill University 1996
Subjects:
Online Access:http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27331
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spelling ndltd-LACETR-oai-collectionscanada.gc.ca-QMM.273312014-02-13T03:46:16ZNuclear regulation of mitochondrial gene expression in Brassica napusHamel, Nancy.Rape (Plant) -- Cytogenetics.Plant genetic regulation.Plant mitochondria.Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act as a dominant gene, designated Mmt, in controlling the production of additional, smaller transcripts of two other mitochondrial loci. The results presented in this thesis reveal that Mmt-specific transcripts lack sequences found at the $5 sp prime$ end of the full-length transcripts of these loci and contain a common sequence, UUGUGG, which maps immediately downstream of their $5 sp prime$ termini. A similar sequence, UUGUUG, is found within orf224 downstream of the major Rfp-specific $5 sp prime$ transcript terminus; these hexanucleotide sequences may serve as recognition motifs in the generation of Mmt- and Rfp-specific transcripts. These results suggest that Rfp/Mmt is a novel nuclear locus affecting the expression of multiple mitochondrial gene regions, with different alleles or haplotypes affecting different mitochondrial genes.McGill UniversityBrown, Gregory G. (advisor)1996Electronic Thesis or Dissertationapplication/pdfenalephsysno: 001564545proquestno: MQ29706Theses scanned by UMI/ProQuest.All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.Master of Science (Department of Biology.) http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27331
collection NDLTD
language en
format Others
sources NDLTD
topic Rape (Plant) -- Cytogenetics.
Plant genetic regulation.
Plant mitochondria.
spellingShingle Rape (Plant) -- Cytogenetics.
Plant genetic regulation.
Plant mitochondria.
Hamel, Nancy.
Nuclear regulation of mitochondrial gene expression in Brassica napus
description Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act as a dominant gene, designated Mmt, in controlling the production of additional, smaller transcripts of two other mitochondrial loci. The results presented in this thesis reveal that Mmt-specific transcripts lack sequences found at the $5 sp prime$ end of the full-length transcripts of these loci and contain a common sequence, UUGUGG, which maps immediately downstream of their $5 sp prime$ termini. A similar sequence, UUGUUG, is found within orf224 downstream of the major Rfp-specific $5 sp prime$ transcript terminus; these hexanucleotide sequences may serve as recognition motifs in the generation of Mmt- and Rfp-specific transcripts. These results suggest that Rfp/Mmt is a novel nuclear locus affecting the expression of multiple mitochondrial gene regions, with different alleles or haplotypes affecting different mitochondrial genes.
author2 Brown, Gregory G. (advisor)
author_facet Brown, Gregory G. (advisor)
Hamel, Nancy.
author Hamel, Nancy.
author_sort Hamel, Nancy.
title Nuclear regulation of mitochondrial gene expression in Brassica napus
title_short Nuclear regulation of mitochondrial gene expression in Brassica napus
title_full Nuclear regulation of mitochondrial gene expression in Brassica napus
title_fullStr Nuclear regulation of mitochondrial gene expression in Brassica napus
title_full_unstemmed Nuclear regulation of mitochondrial gene expression in Brassica napus
title_sort nuclear regulation of mitochondrial gene expression in brassica napus
publisher McGill University
publishDate 1996
url http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27331
work_keys_str_mv AT hamelnancy nuclearregulationofmitochondrialgeneexpressioninbrassicanapus
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