Nuclear regulation of mitochondrial gene expression in Brassica napus
Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act...
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McGill University
1996
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ndltd-LACETR-oai-collectionscanada.gc.ca-QMM.273312014-02-13T03:46:16ZNuclear regulation of mitochondrial gene expression in Brassica napusHamel, Nancy.Rape (Plant) -- Cytogenetics.Plant genetic regulation.Plant mitochondria.Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act as a dominant gene, designated Mmt, in controlling the production of additional, smaller transcripts of two other mitochondrial loci. The results presented in this thesis reveal that Mmt-specific transcripts lack sequences found at the $5 sp prime$ end of the full-length transcripts of these loci and contain a common sequence, UUGUGG, which maps immediately downstream of their $5 sp prime$ termini. A similar sequence, UUGUUG, is found within orf224 downstream of the major Rfp-specific $5 sp prime$ transcript terminus; these hexanucleotide sequences may serve as recognition motifs in the generation of Mmt- and Rfp-specific transcripts. These results suggest that Rfp/Mmt is a novel nuclear locus affecting the expression of multiple mitochondrial gene regions, with different alleles or haplotypes affecting different mitochondrial genes.McGill UniversityBrown, Gregory G. (advisor)1996Electronic Thesis or Dissertationapplication/pdfenalephsysno: 001564545proquestno: MQ29706Theses scanned by UMI/ProQuest.All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.Master of Science (Department of Biology.) http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27331 |
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Rape (Plant) -- Cytogenetics. Plant genetic regulation. Plant mitochondria. |
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Rape (Plant) -- Cytogenetics. Plant genetic regulation. Plant mitochondria. Hamel, Nancy. Nuclear regulation of mitochondrial gene expression in Brassica napus |
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Previous studies have shown that transcriptional differences in the orf224-atp6 mitochondrial gene region are correlated with fertility restoration of the pol CMS trait by the dominant nuclear Rfp gene in Brassica napus. Recently, the recessive rfp allele, or a tightly linked gene, was found to act as a dominant gene, designated Mmt, in controlling the production of additional, smaller transcripts of two other mitochondrial loci. The results presented in this thesis reveal that Mmt-specific transcripts lack sequences found at the $5 sp prime$ end of the full-length transcripts of these loci and contain a common sequence, UUGUGG, which maps immediately downstream of their $5 sp prime$ termini. A similar sequence, UUGUUG, is found within orf224 downstream of the major Rfp-specific $5 sp prime$ transcript terminus; these hexanucleotide sequences may serve as recognition motifs in the generation of Mmt- and Rfp-specific transcripts. These results suggest that Rfp/Mmt is a novel nuclear locus affecting the expression of multiple mitochondrial gene regions, with different alleles or haplotypes affecting different mitochondrial genes. |
author2 |
Brown, Gregory G. (advisor) |
author_facet |
Brown, Gregory G. (advisor) Hamel, Nancy. |
author |
Hamel, Nancy. |
author_sort |
Hamel, Nancy. |
title |
Nuclear regulation of mitochondrial gene expression in Brassica napus |
title_short |
Nuclear regulation of mitochondrial gene expression in Brassica napus |
title_full |
Nuclear regulation of mitochondrial gene expression in Brassica napus |
title_fullStr |
Nuclear regulation of mitochondrial gene expression in Brassica napus |
title_full_unstemmed |
Nuclear regulation of mitochondrial gene expression in Brassica napus |
title_sort |
nuclear regulation of mitochondrial gene expression in brassica napus |
publisher |
McGill University |
publishDate |
1996 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27331 |
work_keys_str_mv |
AT hamelnancy nuclearregulationofmitochondrialgeneexpressioninbrassicanapus |
_version_ |
1716638667057922048 |