Expression studies on the shortbranched chain acyl-CoA dehydrogenase (SBCAD) gene

• Main Author: Vicanek, Caroline Michaela
• Other Authors: Rozen, Rima (advisor)
• Format: Others
• Language: en
• Published: McGill University 1995
• Subjects: Dehydrogenases; Gene expression; Branched chain amino acids > Metabolism > Disorders
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22824

Short/branched chain acyl-CoA dehydrogenase (SBCAD), a member of the acyl-CoA dehydrogenase (ACD) family of enzymes, catalyzes the oxidation of branched chain fatty acids and the branched chain amino acids isoleucine and valine. This research project focuses on expression studies of the SBCAD gene. Northern blot analysis detected two SBCAD mRNA species of 2.7 and 6.5 kb in various human tissues and cell types. A single 4.1 and 2.0 kb SBCAD message was detected in rat and pig tissues, respectively, revealing a species difference in SBCAD mRNA size. Studies of human and rat SBCAD tissue-specificity and relative abundance, at both the RNA and protein levels, identified liver and kidney as the tissues with the highest levels of SBCAD expression, establishing a unique tissue-specific expression pattern that is not seen among the other members of the ACD family. Furthermore, a fetal and adult difference in SBCAD expression was observed in human kidney, suggesting that the SBCAD gene may be developmentally regulated in some tissues. Finally, an attempt was made to isolate and characterize the SBCAD promoter region in order to provide valuable data for future SBCAD promoter studies.