Detection of markers in a low density region of the barley (Hordeum vulgare L.) genome and their effects on the mapping of quantitative traits

• Main Author: Campeol, Nadia.
• Other Authors: Fortin, M. G. (advisor)
• Format: Others
• Language: en
• Published: McGill University 1998
• Subjects: Barley > Molecular genetics.; Plant genome mapping.; Quantitative genetics.
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20809

A modification of bulked segregant analysis was used to raise the density of markers in a 34.5 cM region between Ugp2 and Ugp1, on chromosome 3 of the Harrington x TR306 barley Hordeum vulgare L.) cross. A computer program was used to select pools contrasting for parental alleles at the target site. Of 257 RAPD primers tested on DNA pools, one, UBC 508, detected a polymorphic DNA fragment (UBC508(C)). It mapped 10.2 cM distal to Ugp2. Two additional DNA polymorphisms, (UBC508(A) and UBC508(B)), mapped on chromosome 2. An additional marker, BCD 1796B, mapped 4.9 cM proximal to Ugp1. Both strands of the UBC508(C) fragment were sequenced. They were 588 bp long and had some homology to a region of the DNA that regulates transcription of the H. vulgare pazx gene encoding protein Zx. The effect of adding new marker(s) on the QTL analysis of agronomic and quality traits of barley, was investigated. For extract beta-glucan, a new peak was identified in the analysis when only UBC508(C) or when both UBC508(C) and BCD1796B were added. For fine-coarse difference a QTL x E interaction peak was detected when only BCD 1796B was added or when both UBC508(C) and BCD 1796B were added.