Characterization of the Interactome of the Hippo Tumour Suppressor Pathway using Mass Spectrometry

• Main Author: Yuan, Fang
• Other Authors: Wrana, Jeffrey
• Language: en_ca
• Published: 2013
• Subjects: hippo pathway; mass spectrometry; 0307
• Online Access: http://hdl.handle.net/1807/43372

The Hippo signaling pathway offers an intrinsic mechanism to control organ sizes, and dysfunction of this pathway can often lead to cancer. Great advancement has been made in recent years into understanding this pathway. Despite all this invaluable knowledge, much remains to be explored. Mass spectrometry offers an unbiased approach to characterize the interactome of any protein of interest and is particularly powerful for identifying potential novel regulators of signalling pathways. I therefore set out to characterize the interactome of all the Hippo pathway main components using mass spectrometry, with the goal of uncovering novel regulatory mechanism(s) of the Hippo pathway. In the end, I was able to identify over 250 novel interactors of the Hippo pathway in total. This study demonstrates the utility of mass spectrometry to identify novel regulators of the Hippo pathway and characterization of one such interactor.