Characterization of Hydrolytic Dehalogenases: Substrate Specificity and Isotope Fractionation
The first project is focused on kinetic analysis of two enzymes: Rsc1362 (Ralstonia solanacearum GMI1000) and PA0810 (Pseudomonas aeruginosa PA01). Rsc1362 had a kcat of 504±66 min-1 and a KM of 0.06±0.02 mM, PA0810 had a kcat of 2.6±0.6 min-1 and a KM of 0.44±0.2 mM. A lack of environmental contex...
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| Language: | en_ca |
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2013
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| Online Access: | http://hdl.handle.net/1807/35696 |