In vitro and In vivo High-throughput Analysis of Protein:DNA Interactions

In vitro and In vivo High-throughput Analysis of Protein:DNA Interactions

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In this thesis, emphasis has been placed on development of new approaches for high-throughput analysis of protein:DNA interactions in vitro and in vivo. In vitro strategies for detection of protein:DNA interaction require isolation of active and soluble protein. However, current methodologies for pu...

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Bibliographic Details
Main Author: Shahravan, Seyed Hesam
Other Authors: Shin, Jumi A.
Language:en_ca
Published: 2012
Subjects:
transcription factors
protein:DNA interactions
Yeast hybrid assays
Fluorescent proteins
Enterokinase
proteolysis specificity
DNA-binding proteins
bZIP
Affinity tag
Tag removal
Protein purification
Bioprobe
0486
0487
0491
Online Access:http://hdl.handle.net/1807/33862

Internet

http://hdl.handle.net/1807/33862

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