Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels

Spinal cord injury (SCI) is a devastating condition characterized by the loss of neuronal pathways responsible for coordinating motor and sensory information between the brain and the rest of the body. The mammalian spinal cord is limited in its ability to repair itself, so treatments devised to rep...

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Bibliographic Details
Main Author: Kim, Howard
Other Authors: Shoichet, Molly
Language:en_ca
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/1807/31804
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spelling ndltd-LACETR-oai-collectionscanada.gc.ca-OTU.1807-318042013-04-17T04:19:17ZDirected Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan ChannelsKim, Howardneural stem cellspinal cord injuryguidance channelchitosandrug delivery05410317Spinal cord injury (SCI) is a devastating condition characterized by the loss of neuronal pathways responsible for coordinating motor and sensory information between the brain and the rest of the body. The mammalian spinal cord is limited in its ability to repair itself, so treatments devised to replace damaged tissue and promote regeneration are essential towards developing a cure. This work describes the development of a guidance channel strategy for spinal cord transection. Chitosan guidance channels were designed as a delivery vehicle for neural stem/progenitor cell (NSPC) transplants and drug-eluting poly(lactic-co-glycolic acid) (PLGA) microspheres. PLGA microspheres were embedded into chitosan channels by a spin-coating method. These microsphere-loaded channels demonstrated the ability for controlled short-term bioactive release of the small molecule drug dibutyryl cyclic-AMP (dbcAMP) and long-term bioactive release of the protein alkaline phosphatase. NSPCs were shown to be responsive to dbcAMP delivery, which results in greatly enhanced differentiation into neurons. The effect of directed neuronal differentiation was investigated after spinal cord transection in rat, resulting in a dramatic increase in NSPC transplant survival. Guidance channels containing NSPCs treated with dbcAMP resulted in robust tissue bridge formation after SCI, demonstrating extensive axonal regeneration and promoting functional recovery.Shoichet, MollyTator, Charles2011-112012-01-10T15:25:29ZNO_RESTRICTION2012-01-10T15:25:29Z2012-01-10Thesishttp://hdl.handle.net/1807/31804en_ca
collection NDLTD
language en_ca
sources NDLTD
topic neural stem cell
spinal cord injury
guidance channel
chitosan
drug delivery
0541
0317
spellingShingle neural stem cell
spinal cord injury
guidance channel
chitosan
drug delivery
0541
0317
Kim, Howard
Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
description Spinal cord injury (SCI) is a devastating condition characterized by the loss of neuronal pathways responsible for coordinating motor and sensory information between the brain and the rest of the body. The mammalian spinal cord is limited in its ability to repair itself, so treatments devised to replace damaged tissue and promote regeneration are essential towards developing a cure. This work describes the development of a guidance channel strategy for spinal cord transection. Chitosan guidance channels were designed as a delivery vehicle for neural stem/progenitor cell (NSPC) transplants and drug-eluting poly(lactic-co-glycolic acid) (PLGA) microspheres. PLGA microspheres were embedded into chitosan channels by a spin-coating method. These microsphere-loaded channels demonstrated the ability for controlled short-term bioactive release of the small molecule drug dibutyryl cyclic-AMP (dbcAMP) and long-term bioactive release of the protein alkaline phosphatase. NSPCs were shown to be responsive to dbcAMP delivery, which results in greatly enhanced differentiation into neurons. The effect of directed neuronal differentiation was investigated after spinal cord transection in rat, resulting in a dramatic increase in NSPC transplant survival. Guidance channels containing NSPCs treated with dbcAMP resulted in robust tissue bridge formation after SCI, demonstrating extensive axonal regeneration and promoting functional recovery.
author2 Shoichet, Molly
author_facet Shoichet, Molly
Kim, Howard
author Kim, Howard
author_sort Kim, Howard
title Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
title_short Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
title_full Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
title_fullStr Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
title_full_unstemmed Directed Adult Neural Stem/Progenitor Cell Fate in Microsphere-loaded Chitosan Channels
title_sort directed adult neural stem/progenitor cell fate in microsphere-loaded chitosan channels
publishDate 2011
url http://hdl.handle.net/1807/31804
work_keys_str_mv AT kimhoward directedadultneuralstemprogenitorcellfateinmicrosphereloadedchitosanchannels
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