Translational Regulation of smaug mRNA

• Main Author: Votruba, Melissa
• Other Authors: Lipshitz, Howard
• Language: en_ca
• Published: 2009
• Subjects: Translation; mRNA; Pumilio; Smaug; Drosophila; maternal mRNA; oocyte; embryo; 0369; 0307
• Online Access: http://hdl.handle.net/1807/29981

In Drosophila, early embryonic development is controlled by maternally loaded RNAs and proteins. For proper development to occur it is vital these maternal transcripts are post-transcriptionally regulated. SMAUG, a major post-transcriptional regulator, has been found to be responsible for the destabilization of two thirds of the unstable maternal transcripts upon egg activation (Tadros et al., 2007). smg mRNA is translationally repressed in stage 14 oocytes, but its translation is activated upon egg activation in a PAN GU kinase dependent manner. Here I show that redundant translational repression elements reside in the smg 3’UTR, and PUMILIO mediates repression through one of these elements. I also show that these elements are sufficient to cause translational repression in stage 14 oocytes. smg mRNA appears to be regulated post-initiation in stage 14 oocytes in a large repression complex which is similar to smg mRNA repression in a png mutant.