Developing experimental methods for identifying the sites of action of intraspinal microstimulation

Intraspinal microstimulation (ISMS) is a novel electrical stimulation approach to restore standing and ambulation in people with spinal cord injury. The technique entails inserting an array of microwires into the lumbosacral enlargement of the spinal cord to activate neuronal networks that control...

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Main Author: Christian, Breanne
Other Authors: Mushahwar, Vivian (Cell Biology)
Format: Others
Language:en_US
Published: 2011
Online Access:http://hdl.handle.net/10048/1938
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spelling ndltd-LACETR-oai-collectionscanada.gc.ca-AEU.10048-19382012-03-21T22:50:08ZMushahwar, Vivian (Cell Biology)Christian, Breanne2011-05-17T15:23:25Z2011-05-17T15:23:25Z2011-05-17T15:23:25Zhttp://hdl.handle.net/10048/1938Intraspinal microstimulation (ISMS) is a novel electrical stimulation approach to restore standing and ambulation in people with spinal cord injury. The technique entails inserting an array of microwires into the lumbosacral enlargement of the spinal cord to activate neuronal networks that control locomotion. Additionally, ISMS can be utilized to investigate the organization of these networks. In the present study, experimental methodology was developed to map the distribution of ISMS-activated neurons using immunohistochemistry to label c-Fos, an activity-dependent marker. The influence on c-Fos expression of the following conditions was studied: decerebration, laminectomy, microwire implantation, and ISMS. Data revealed that microwire implantation and decerebration minimally influenced c-Fos, while a laminectomy substantially increased c-Fos expression. Furthermore, results indicated that it is vital to monitor stimulation and adjust stimulus amplitude throughout the duration of stimulation. Using these data, a protocol was established that would aid in mapping the ISMS activated neuronal networks.6012011 bytesapplication/pdfen_USDeveloping experimental methods for identifying the sites of action of intraspinal microstimulationThesisMaster of ScienceMaster'sCell BiologyUniversity of Alberta2011-11Campenot, Bob (Cell Biology)Todd, Kathryn (Psychiatry)Krukoff, Teresa (Cell Biology)
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language en_US
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description Intraspinal microstimulation (ISMS) is a novel electrical stimulation approach to restore standing and ambulation in people with spinal cord injury. The technique entails inserting an array of microwires into the lumbosacral enlargement of the spinal cord to activate neuronal networks that control locomotion. Additionally, ISMS can be utilized to investigate the organization of these networks. In the present study, experimental methodology was developed to map the distribution of ISMS-activated neurons using immunohistochemistry to label c-Fos, an activity-dependent marker. The influence on c-Fos expression of the following conditions was studied: decerebration, laminectomy, microwire implantation, and ISMS. Data revealed that microwire implantation and decerebration minimally influenced c-Fos, while a laminectomy substantially increased c-Fos expression. Furthermore, results indicated that it is vital to monitor stimulation and adjust stimulus amplitude throughout the duration of stimulation. Using these data, a protocol was established that would aid in mapping the ISMS activated neuronal networks.
author2 Mushahwar, Vivian (Cell Biology)
author_facet Mushahwar, Vivian (Cell Biology)
Christian, Breanne
author Christian, Breanne
spellingShingle Christian, Breanne
Developing experimental methods for identifying the sites of action of intraspinal microstimulation
author_sort Christian, Breanne
title Developing experimental methods for identifying the sites of action of intraspinal microstimulation
title_short Developing experimental methods for identifying the sites of action of intraspinal microstimulation
title_full Developing experimental methods for identifying the sites of action of intraspinal microstimulation
title_fullStr Developing experimental methods for identifying the sites of action of intraspinal microstimulation
title_full_unstemmed Developing experimental methods for identifying the sites of action of intraspinal microstimulation
title_sort developing experimental methods for identifying the sites of action of intraspinal microstimulation
publishDate 2011
url http://hdl.handle.net/10048/1938
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