Nuclear Magnetic Resonance metabolomic fingerprint of the Interleukin 10 gene deficient mouse model of Inflammatory Bowel Disease

• Main Author: Tso, Victor Key
• Other Authors: Fedorak, Richard (Medicine)
• Format: Others
• Language: en
• Published: 2010
• Subjects: NMR; Metabolomics; Inflammatory Bowel Disease; Metabolite; IL 10; Mouse; Germ free
• Online Access: http://hdl.handle.net/10048/1450

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder that occurs as a consequence of a genetic mutation that results in an overly aggressive immune response to normal bacteria. Metabolomics is a new born cousin to genomics and proteomics and involves a high throughput identification, characterization and quantification of small molecule metabolites generated by the organism. This study will show that metabolomics can be an effective tool in studying the differences between wild type and IL 10 KO mice as they age in axenic and conventional environments, and the onset of disease in a conventional environment. I show specific changes upon colonizing axenic mice with fecal bacteria that are similar to changes occurring over 16 weeks of conventional growth. Several bacterial metabolites have been identified that may play a role in the pathogenesis or provide clues to the interactions of the gut microbiota with the intestinal immune system. === Experimental Medicine