A directed evolution design of target specificity and kinetic analysis of conformational transitions in the HhaI methyltransferase
DNA cytosine-5 methyltransferases (MTases) recognize short DNA sequences and catalyze the transfer of the methyl group from the cofactor AdoMet to the C5-position of a target cytosine. In this work both these aspects of the MTase mechanism have been addressed. First, using rational protein design an...
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| Format: | Doctoral Thesis |
| Language: | English |
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Lithuanian Academic Libraries Network (LABT)
2011
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| Online Access: | http://vddb.laba.lt/fedora/get/LT-eLABa-0001:E.02~2011~D_20110519_082240-87105/DS.005.1.01.ETD |