| Summary: | The goal of our research was to optimize the conditions of chromatographic analysis of penicillin, to define minimal found analysis concentration, analyse contaminated milk samples with given certain concentration and establish resumption of penicillin G. Therefore, we aimed at analysing milk samples‘ contamination with penicillin of randomly selected cows from study farms.
The research was performed in Lithuanian Academy of Veterinary which has a practical training and experiment centre in Muniskes cow farm, state enterprise „Pieno tyrimai“ and Food Institute of Kaunas Technological University.
State enterprise „Pieno tyrimai“ used preparation LPT to define milk inhibitors. After the bacteriological milk samples analyses 8 out of 25 samples were found to portray remnants of inhibitory materials.
Optimal chromatographic conditions were established by the use of experiment, in accordance with a thorough analysis of literature concentrating on chromatographic methods dealing with penicillin G determination. The following are defined conditions for the chromatographic examination of penicillin G: composition of a mobile phaze 0 – 10 min 5% ACN / 95% 0,125 M phosphate buffer (pH=3,5), 12-22 min 70 % ACN / 30% 0,125 M phosphate buffer, 24-30 min 5% ACN / 95% 0,125 M phosphate buffer (gradient flow mode). Period of assay suspention was about 17 minutes. Resumption of contaminated milk samples with penicillin G 500 mg/kg, 250 mg/kg, 50 mg/kg and 4 mg/kg varied from 45 to 76 %. The... [to full text]
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