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Previous issue date: 2017-09-29 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES === The genus Hyptis is composed of about 400 species distributed throughout several countries, the majority being in a native and non-domesticated state. The genus has great importance for the pharmaceutical industry because of the high diversity found in its species. It has excellent pharmacological potential and is of great economic interest. Biological properties like it?s antimicrobial, anti - inflammatory, anesthetic, and insecticidal potential have already been demonstrated. However, in addition to the endemism present in many species, there are few works related to conservation of the genus and more research is needed to elucidate potential methods for its preservation. The objective of this research was the in vitro conservation of the species Hyptis ramosa. In addition to adjusting the protocols of cryopreservation of axillary guides and shoots, we aimed to contribute to the conservation of germplasm of this species. The microplants used came from the germination of seeds in MS1/2 medium. For the in vitro conservation, 10 treatments were evaluated, with different combinations between sucrose, mannitol, and sorbitol. After inoculation, the plants were maintained in a growth room for 240 days and evaluated at 60, 120, 180, and 240 days. Each evaluation quantified the number of shoots, shoot length, root number, and root length. For cryopreservation, the techniques of vitrification for axillary calluses and buds and for encapsulation of axillary shoots were evaluated. The tests were carried out in the Laboratory of Germination (LAGER) and Vegetable Tissue Culture (LCTV) of the Horto Florestal Experimental Unit da Universidade Estadual de Feira de Santana. With the results obtained, we concluded that in vitro conservation of microplants of this species is possible using the combination of 87.64 mM sucrose combined with 87.64 mM mannitol in MS medium (MURASHIGE; SKOOG, 1969). With the methodology used, cryopreservation of calluses and shoots of the species was not possible. Cell death occurred in the first stages of the callus vitrification process and in the cryogenic stage for the shoots. === O g?nero Hyptis abrange cerca de 400 esp?cies distribu?das em v?rios pa?ses, sendo a grande maioria ainda encontrada em estado nativo e n?o domesticado. O g?nero apresenta grande import?ncia para a ind?stria farmac?utica por apresentar uma grande diversidade de esp?cies com grande potencial farmacol?gico e de interesse econ?mico, com diversas atividades biol?gicas j? comprovadas, como antimicrobiana, anti-inflamat?ria, anest?sica e inseticida. Contudo, al?m do endemismo presente em muitas esp?cies existem poucos trabalhos relacionados com a sua conserva??o, havendo a necessidade de mais pesquisas. No presente trabalho objetivou-se a conserva??o in vitro de plantas de Hyptis ramosa, al?m de ajustar protocolos para crioconserva??o de calos e gemas axilares, visando contribuir para a conserva??o de germoplasma desta esp?cie. As microplantas utilizadas foram provenientes da germina??o de sementes em meio MS1/2. Para conserva??o in vitro foram avaliados 10 tratamentos, com diferentes combina??es entre sacarose, manitol e sorbitol. Ap?s a inocula??o as plantas foram mantidas em sala de crescimento por 240 dias, com avalia??es aos 60, 120, 180 e 240 dias, quantificando-se o n?mero de brotos, comprimento da parte a?rea, n?mero de raiz e comprimento da raiz. Para a crioconserva??o foram avaliadas as t?cnicas de vitrifica??o para calos e gemas axilares e de encapsulamento de gemas axilares. Os ensaios foram realizados nos Laborat?rios de Germina??o (LAGER) e de Cultura de Tecidos Vegetais (LCTV) da Unidade Experimental Horto Florestal da Universidade Estadual de Feira de Santana. Com os resultados obtidos p?de-se concluir que ? poss?vel a conserva??o in vitro de microplantas dessa esp?cie, utilizando-se a combina??o de 87,64mM de sacarose combinado com 87,64mM de manitol, em meio MS (MURASHIGE; SKOOG, 1969). Com a metodologia utilizada n?o foi poss?vel a crioconserva??o de calos e gemas da esp?cie, havendo a morte celular nas primeiras etapas do processo de vitrifica??o dos calos e na etapa criog?nica para as gemas.
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