Indu??o de calos em anteras e poliploidia em gen?tipos de melancia

• Main Author: Silva, Carla Maria de Jesus
• Other Authors: Melo, Natoniel Franklin de
• Format: Others
• Language: Portuguese
• Published: Universidade Estadual de Feira de Santana 2018
• Subjects: Calog?nese em anteras; Citogen?tica; Citrullus lanatus; Gametas n?o reduzidos; Melancia sem semente; Viabilidade pol?nica em melancia; Calogenesis in anthers; Cytogenetics; Unreduced gametes; Seedless watermelon; Pollen viability in watermelon; GENETICA::GENETICA VEGETAL
• Online Access: http://tede2.uefs.br:8080/handle/tede/tede/631

Submitted by Verena Pereira ([e-mail]) on 2018-07-03T23:23:34Z No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5) === Made available in DSpace on 2018-07-03T23:23:34Z (GMT). No. of bitstreams: 1 TESE 2018 - Doutorado RGV_UEFS - Carla Maria de Jesus Silva.pdf: 1687907 bytes, checksum: 3e9fe14e189c00f951079ca2a71e3c66 (MD5) Previous issue date: 2018-03-29 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES === The watermelon (Citrullus lanatus) is a very important vegetable for a region of Northeast Brazil due to its adaptation as the natural conditions and the good characteristics of the fruit that are obtained. The aims of the current study are to assess watermelon genotype responses to calluses in anthers by using growth and temperature regulators, and to induce polyploidy through colchicine use (at different concentrations), exposure time, mechanical scarification and application methods. Anthers of Smile and Sugar Baby lines were inoculated in MS medium at different concentrations of 2.4-D or of BAP with 2.4-D, in combination with the pre-treatment (low temperature). Crimson Sweet cultivar seedlings were treated with different colchicine concentrations at two different times, with and without scarification, in order to induce polyploidy. Line LDRO was subjected to different colchicine concentrations at two different times and application methods: a) direct in the seed method (with, and without, scarification) (DSM, WE and WOE), b) Radicle emission method (ERM), c) Hypocotyl and root insertion point method (HRIM), d) At the apex of the seedling method (ASM) and e) Inverted hypocotyl method (IHM). Crimson Sweet flower buds were subjected to colchicine, at two different times, in order to induce polyploidy. Results have shown that 2.4-D often induced callus formation in both lines, but BAP/2.4-D interaction and pre-treatment did not increase the induction frequency. Crimson Sweet showed higher induction rate at 0.2% colchicine for 48h, WE. Line LDRO presented plants with tetraploid cells at 0.2% colchicine for 24 and 48h. The method 0.2% DSM, WE and WOE also generated plants with tetraploid cells. The diameter of treated pollen grains in flower buds have increased; the higher rate of non-reduced gametes induction was 16.07% and flower bud diameter (1.5mm) was estimated as adequate for induction === A melancia (Citrullus lanatus) ? uma hortali?a muito importante para a regi?o Nordeste do Brasil por sua adapta??o as condi??es clim?ticas e pelas boas caracter?sticas de fruto que se obt?m. Os objetivos desse trabalho foram avaliar as respostas de gen?tipos de melancia quanto ? indu??o de calos em anteras utilizando reguladores de crescimento e temperatura; e induzir a poliploidia mediante o uso da colchicina em diferentes concentra??es, tempos de exposi??o, escarifica??o mec?nica e m?todos de aplica??o. Anteras das linhagens de Smile e Sugar Baby foram inoculadas em meio MS sob diferentes concentra??es de 2,4-D ou BAP com 2,4-D, associado ao pr?-tratamento (baixa temperatura). Para indu??o de poliploidia, sementes da cultivar Crimson Sweet foram tratadas com diferentes concentra??es de colchicina, em dois tempos, com e sem escarifica??o. Para a linhagem LDRO, utilizaram-se diferentes concentra??es de colchicina em dois tempos e m?todos de aplica??o: a) M?todo direto na semente (com escarifica??o e sem escarifica??o) (MDS, CE e SE), b) M?todo da semente com emiss?o da rad?cula (MER), c) M?todo no ponto de inser??o do hipoc?tilo e raiz (MIHR), d) M?todo no ?pice da pl?ntula (MAP) e e) M?todo do hipoc?tilo invertido (MHI). Na indu??o de poliploidia em bot?es florais de Crimson Sweet utilizou-se colchicina em dois tempos. Os resultados mostraram que o 2,4-D induziu a maior frequ?ncia de calos nas duas linhagens, a intera??o BAP com 2,4-D e o pr?-tratamento, n?o aumentaram a frequ?ncia de indu??o. Para Crimson Sweet, o maior percentual de indu??o foi obtido com colchicina a 0,2% por 48 h, SE. Para LDRO, observou-se uma frequ?ncia de plantas com c?lulas tetraploides com colchicina a 0,2% por 24 h e 48 h. Nos m?todos 0,2% MDS CE e MHI observou-se tamb?m plantas com c?lulas tetraploides. Em bot?es florais, o di?metro dos gr?os de p?len tratados aumentou; o maior percentual de indu??o de gametas n?o reduzidos foi de 16,07% e; o di?metro em torno de 1,5 mm do bot?o floral foi estimado como adequado para indu??o