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Previous issue date: 2017-02-15 === The use of selected yeasts is an important aspect in the production of quality beers, since in addition to the production of ethanol and carbon dioxide they contribute significantly to the formation of secondary compounds, which confer the flavor of the beer. These yeasts also settle easily in the middle, avoiding undesired contaminations, in addition to generally presenting high rates of yield, productivity and fermentation efficiency. Selenium (Se) is a mineral that performs antioxidant functions, being essential as trace element to human health and its deficiency is correlated to the occurrence of several diseases. Due to these properties, the incorporation of Se into some food and / or food supplements associated with yeast is already a reality. Considering that beer is the most consumed alcoholic drink in the world, this research aimed to select yeasts to produce artisan beer supplemented with selenium. To do so, using the multivariate analysis technique, the selection of yeasts with potential for brewing was initially performed, and three strains (SC52, SC57 and SC82) were selected; Then applying the Doehlert factorial design and using the Response Surface Methodology technique, the optimization of the fermentation parameters (fermentation temperature and selenium concentration) for the production of beer supplemented with selenium was carried out. The yeasts SC52 and SC82 presented significant responses for the production of the supplemented beer, and the optimum values (Se concentration and fermentation temperature) were 11 mg L-1 and 25.0? C for yeast SC52 and 12 mg L-1 and 23.5? C for yeast SC82; (CSeSC52 and CSeSC82) were produced on a microbrewery scale, the fermentation parameters of the process were evaluated and total selenium and selenium species present in yeast and beers were quantified. The beers produced presented fermentative results within the commercial standards, being recommended by DDR (Recommended Daily Dose) of Se, a maximum consumption of approximately 600 mL of CSeSC52 beer and 740 mL of CSeSC82 beer. === O uso de leveduras selecionadas constitui aspecto relevante na produ??o de cervejas de qualidade, pois al?m da produ??o de etanol e g?s carb?nico contribuem significativamente para a forma??o de compostos secund?rios, os quais conferem o flavor da cerveja. Estas leveduras tamb?m se estabelecem facilmente no meio, evitando contamina??es indesejadas, al?m de apresentarem, geralmente, altas taxas de rendimento, produtividade e efici?ncia na fermenta??o. O sel?nio (Se) ? um mineral que desempenha fun??es antioxidantes, sendo essencial como elemento tra?o ? sa?de humana e a sua defici?ncia est? correlacionada ? ocorr?ncia de v?rias doen?as. Devido a estas propriedades, a incorpora??o do Se em alguns alimentos e/ou suplementos alimentares associados a leveduras j? ? uma realidade. Considerando que a cerveja constitui a bebida alco?lica mais consumida no mundo, esta pesquisa objetivou selecionar leveduras para produzir cerveja artesanal suplementada com sel?nio. Para tanto, a partir do uso da t?cnica de an?lise multivariada, inicialmente foi realizada a sele??o de leveduras com potencial para produ??o de cerveja, sendo selecionadas tr?s cepas (SC52, SC57 e SC82); em seguida, aplicando o planejamento fatorial Doehlert e utilizando a t?cnica de Metodologia de Superf?cie de Respostas, realizou-se a otimiza??o dos par?metros fermentativos (temperatura de fermenta??o e concentra??o de sel?nio) para a produ??o de cerveja suplementada com sel?nio. As leveduras SC52 e SC82 apresentaram respostas significativas para a produ??o da cerveja suplementada, sendo que os valores ?timos (concentra??o de Se e Temperatura de fermenta??o) obtidos foram de 11 mg L-1 e 25,0 ?C para a levedura SC52 e 12 mg L-1 e 23,5 ?C para a levedura SC82; por fim, foram produzidas duas cervejas artesanais suplementadas com sel?nio (CSeSC52 e CSeSC82) em escala de microcervejaria, sendo avaliados os par?metros fermentativos do processo e quantificados o sel?nio total e as esp?cies de sel?nio presentes nas leveduras e nas cervejas. As cervejas produzidas apresentaram resultados fermentativos dentro dos padr?es comerciais, sendo recomendado em virtude da DDR (Dose Di?ria Recomendada) de Se, um consumo m?ximo de aproximadamente 600 mL da cerveja CSeSC52 e 740 mL da cerveja CSeSC82.
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