O emprego da eletroestimula??o associada ? t?cnica de tubula??o na regenera??o do nervo ci?tico de ratos wistar ap?s transec??o sem perda de subst?ncia

• Main Author: Pit?goras, Tatiana Zotti
• Other Authors: Silva, Jefferson Braga
• Format: Others
• Language: Portuguese
• Published: Pontif?cia Universidade Cat?lica do Rio Grande do Sul 2018
• Subjects: Eletroestimula??o; Regenera??o; Les?o de Nervos Perif?ricos; Tubuliza??o; CIENCIAS DA SAUDE::MEDICINA
• Online Access: http://tede2.pucrs.br/tede2/handle/tede/8213

Submitted by PPG Medicina e Ci?ncias da Sa?de ([e-mail]) on 2018-07-12T13:10:54Z No. of bitstreams: 1 TATIANA_ZOTTI_PIT?GORAS.pdf: 16837328 bytes, checksum: 65a299720852db7830bef151dfdba46a (MD5) === Approved for entry into archive by Sheila Dias ([e-mail]) on 2018-07-17T14:10:24Z (GMT) No. of bitstreams: 1 TATIANA_ZOTTI_PIT?GORAS.pdf: 16837328 bytes, checksum: 65a299720852db7830bef151dfdba46a (MD5) === Made available in DSpace on 2018-07-17T14:31:09Z (GMT). No. of bitstreams: 1 TATIANA_ZOTTI_PIT?GORAS.pdf: 16837328 bytes, checksum: 65a299720852db7830bef151dfdba46a (MD5) Previous issue date: 2018-03-28 === Introduction: Peripheral nerve transection injuries are common and occur frequently in clinical practice and are responsible for serious problems such as pain, loss of sensibility and motor, and permanent sequelae. Great advances were obtained in the treatment of peripheral nerve lesions due to greater knowledge about their physiology and the development of techniques such as microsurgery. The results of electrical stimulation are encouraging and suggest its use as an alternative treatment. Objective: To evaluate, in vivo, the use of electrostimulation (EE) associated with the tubulization technique on the repair of sciatic nerve injury in Wistar rats. Materials and Methods: Ninety male Wistar isogenic rats weighing between 200 and 300 grams were divided into three groups of 30 rats (electrostimulation associated with the tubing technique (EE), tubing suture (ST) and suture(SUT), which were subdivided into three other groups with ten animals according to the evaluation time, with three, six and nine weeks postoperative. Regeneration was assessed by ?walking track? analysis and histological analysis in which determination of the area and number of Schwann cells in the nerve samples. Results: In the functional evaluation, the group that received only one EE with 20 Hz, 3 V for one hour presented a superior result, statistically significant when compared to those stimulated more than once, however when comparing the three different types of treatment at the end of the nine weeks there was statistically significant superiority in the treated with standard suture. Regarding the number of cells, no statistically significant result was found in either group. The findings related to the nerve area, in those submitted to EE at three weeks, when compared to the other groups in the same period, were significant, and in the comparison within the same group, those with suture and tube at six weeks obtained a regenerated nerve with area in a statistically significant way. === Introdu??o: Traumatismos por transec??o de nervo perif?rico s?o comuns e ocorrem, frequentemente, na pr?tica cl?nica, sendo respons?veis por problemas graves como dor, perda de sensibilidade e motricidade, al?m de sequelas permanentes. Grandes avan?os foram obtidos no tratamento das les?es dos nervos perif?ricos, devido ao maior conhecimento sobre sua fisiologia e ao desenvolvimento de t?cnicas como a microcirurgia. Os resultados da estimula??o el?trica s?o encorajadores e sugerem seu uso como alternativa de tratamento. Objetivo: Avaliar, in vivo, o uso da eletroestimula??o (EE) associada ? t?cnica de tubuliza??o sobre o reparo de les?o de nervo ci?tico, em ratos Wistar. Materiais e m?todos: Noventa ratos isog?nicos da ra?a Wistar, machos, adultos, pesando entre 200 e 300 gramas de massa corporal, foram divididos em tr?s grupos de 30 ratos (eletroestimula??o associada ? t?cnica de tubuliza??o (EE), sutura com tubuliza??o (ST) e sutura padr?o (SUT). Os tr?s grupos foram ainda subdivididos em tr?s grupos com 10 animais, de acordo com o tempo de avalia??o (tr?s, seis e nove semanas de p?s-operat?rio). A regenera??o foi avaliada atrav?s da an?lise da marcha e an?lise histol?gica, em que houve a determina??o da ?rea e do n?mero de c?lulas de Schwann nas amostras de nervo. Resultados: Na avalia??o funcional, o grupo que recebeu somente uma EE com 20 Hz e 3V por uma hora apresentou um resultado superior se comparado com os estimulados mais de uma vez. Entretanto, ao se comparar os tr?s diferentes tipos de tratamento ao final das nove semanas, os ratos com sutura padr?o apresentaram resultados melhores. Com rela??o ao n?mero de c?lulas, n?o foi encontrado resultado estatisticamente significativo em nenhum dos grupos. Em rela??o aos achados relacionados ? ?rea do nervo, naqueles submetidos ? EE com tr?s semanas, quando comparados aos outros grupos no mesmo per?odo, houve signific?ncia, sendo que na compara??o dentro do mesmo grupo, aqueles com sutura e tubo com seis semanas, obtiveram um nervo regenerado com ?rea maior.