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Previous issue date: 2014-12-19 === Objective: To evaluate the bone formation with different bone substitutes, including a rhGH hormone carrier (Growth Human Hormone Recombined) in an animal model. Materials and Methods: A total of 12 rabbits (from New Zealand), males, young adults (approximately 10 months old) were used. For each group, 6 rabbits were used, being that in each animal, 4 test sites were performed with a 8 mm diameter trephine, completing the total thickness of the skull. The test sites were distributed in: Group I (a: clot, b: autogenous bone triturated, c: PLGA (poly lactic glycolic acid) + rhGH (Growth Human Hormone Recombined), and d: autogenous bone + rhGH); and Group II (a: HA (hydroxyapatite), b: autogenous bone triturated, c: HA / ?-TCP (hydroxyapatite / tricalcium phosphate beta), and d: HA / SiO2n (hydroxyapatite / silicium oxide). The animals were sacrificed after 6 weeks and the samples were prepared and stained with HE (hematoxylin and eosin). A descriptive analysis of the samples was performed with OM (optics microscopic). Results: The evaluation showed that in the samples with PLGA and rhGH there was not the complete filling of the sites, although it was observed inflammatory process compatible with existing biomaterials. The bone formation regions presented filled
osteocytic gaps in remodeling process, showing mature and viable bone. In relation to the sites with biomaterials (HA, HA/TCP-b e HA/ HA/SiO2n), all of them showed bone formation with biomaterial incorporation. Conclusion: The results of this study demonstrated the viability of different bone substitutes (HA, HA / TCP-?, HA / SiO2n). In addition, bone formation was not observed throughout the test region with the use of the hormone carrier. However, in regions with neoformation, it was verified the presence of mature and viable bone. === Objetivo: Avaliar a forma??o ?ssea com diferentes substitutos ?sseos, incluindo um carreador de horm?nio rhGH (horm?nio do crescimento) em defeitos cr?ticos em um modelo animal. Materiais e M?todos: Foram utilizados 12 coelhos da ra?a Nova Zel?ndia, machos, e adultos jovens (aproximadamente 10 meses). Para cada grupo foi utilizado 6 coelhos, sendo que em cada animal foram realizadas 4 cavidades teste com broca
trefina de 8mm de di?metro e espessura total da calota craniana. As cavidades teste foram distribu?das em: Grupo I (a: co?gulo, b: osso aut?geno triturado, c: PLGA (Poli ?cido glic?lico l?tico) + rhGH (horm?nio do crescimento recombinante) e, d: osso aut?geno triturado + rhGH) e Grupo II (a: HA (hidroxiapatita), b: osso aut?geno triturado, c: HA/TCP-? (hidroxiapatita/tric?lcio fosfato beta) e d: HA/SiO2n (hidroxiapatita/?xido de sil?cio). Os animais foram sacrificados ap?s 6 semanas, e as amostras foram preparadas e coradas com colora??o HE (hematoxilina e eosina). Foi realizada uma an?lise descritiva das amostras por MO (microscopia ?ptica). Resultados: A avalia??o mostrou que nas amostras com PLGA e rhGH n?o houve o
preenchimento total da cavidade, embora tenha sido observado neoforma??o ?ssea. Essas regi?es apresentavam-se com as lacunas osteoc?ticas preenchidas e em processo de remodela??o, evidenciando osso maduro e vi?vel. Com rela??o aos biomateriais (HA, HA/TCP-? ? ??/ HA/SiO2n), todos apresentaram forma??o ?ssea com a incorpora??o do biomaterial. Conclus?o: Os resultados do presente estudo demostraram a neoforma??o ?ssea com os diferentes substitutos ?sseos (HA, HA/TCP-?, HA/SiO2n). Al?m disso, foi observada a viabilidade do uso do carreador de horm?nio com a presen?a de osso maduro e vi?vel nas regi?es de teste desse.
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