Avalia??o do papel da prote?na STAT3 na infec??o V?rus Sincicial Respirat?rio e resposta de c?lulas T CD8 de mem?ria

• Main Author: Becker, Andr? Luiz
• Other Authors: Renato Tetelbom, Renato Tetelbom
• Format: Others
• Language: Portuguese
• Published: Pontif?cia Universidade Cat?lica do Rio Grande do Sul 2017
• Subjects: VIROSES; MEM?RIA; PEDIATRIA; MEDICINA; CIENCIAS DA SAUDE::MEDICINA
• Online Access: http://tede2.pucrs.br/tede2/handle/tede/7145

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS ([e-mail]) on 2017-03-07T17:56:28Z No. of bitstreams: 1 DIS_ANDRE_LUIZ_BECKER_PARCIAL.pdf: 253481 bytes, checksum: 8161f92176563892b9395f8946b40cb9 (MD5) === Made available in DSpace on 2017-03-07T17:56:28Z (GMT). No. of bitstreams: 1 DIS_ANDRE_LUIZ_BECKER_PARCIAL.pdf: 253481 bytes, checksum: 8161f92176563892b9395f8946b40cb9 (MD5) Previous issue date: 2016-08-31 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES === Introduction: The formation of memory T cells is critical in adaptive immunity, when re-exposure to an antigen, it allows immediate response in this new challenge. This condition is very important in infections, vaccines and cancer. CD8 T cells present specific characteristics among the other lymphocytes regarding the formation of its memory cell populations. Recently the complex mechanism of cytokines and transcription factors that direct to the different phenotypic outcomes has been studied. A relevant factor in this process is the activation of signal transducers to the nucleus (STATs), which activate the transcription of DNA. From this group STAT3 (Signaling Transducer and Activator of Transcription 3) seems to have a crucial role in the formation of memory CD8 T cells. Objectives: To deepen the knowledge about the formation of memory T cells, mostly CD8 T cell, through STAT3 protein in this process. Also to review the literature and expand with original experiments the clinical and laboratory approach of immunity to RSV regarding the CD8-STAT3 relationship. Methods: Review article: A search was made in the database using Boolean operators in PUBMED platform on the relationship between STAT3 and development of CD8 memory T cell subsets. The selected articles were analyzed and summarized. Original article: In vitro experiments were performed to analyze the effect of RSV in STAT3 and its relationship with the different memory CD8 T cell profiles. Results: STAT3 is an important part in defining the profile of memory CD8 T cells and undergoes significant changes during RSV infection. Conclusion: STAT plays a central role in the outcome of the memory CD8 T cell development. STAT3 activation and expression are indeed influenced by RSV infection in human and murine models, which suggests new possibilities for therapeutical interventions. === Introdu??o: A forma??o de c?lulas T de mem?ria ? fundamental na imunidade adaptativa, quando da reexposi??o a um dado ant?geno, pois permite uma resposta praticamente imediata neste novo desafio. Esta condi??o ? muito importante nas infec??es, vacinas e no c?ncer. As c?lulas T CD8 (LTCD8) apresentam caracter?sticas pr?prias dentre os demais linf?citos, tamb?m no tocante a forma??o de suas subpopula??es de c?lulas de mem?ria. O intrincado mecanismo de citocinas e fatores de transcri??o que definem os diferentes fen?tipos resultantes, vem sendo recentemente estudados. Um fator relevante neste processo ? a ativa??o dos transdutores de sinal para o n?cleo (STATs), que ativam a transcri??o do DNA. Deste grupo a STAT3 (Signaling Transducer and Activator of Transcription 3) parece ter papel crucial na forma??o de LTCD8 de mem?ria. Objetivos: Aprofundar o conhecimento sobre a forma??o de c?lulas T de mem?ria, em especial as LTCD8, atrav?s da prote?na STAT3 fundamental neste processo; revisar a literatura conhecida e ampliar com experimentos originais o enfoque cl?nico-laboratorial de imunidade ao VSR no tocante ? rela??o CD8-STAT3.Metodologia: Artigo de revis?o: Foi feita uma busca na base de dados utilizando operadores Booleanos na plataforma PUBMED, sobre a rela??o entre STAT3 e o desenvolvimento das c?lulas T CD8 de mem?ria. Os artigos foram analisados e resumidos neste trabalho. Artigo original: Foi realizada uma an?lise in vitro sobre o efeito do VSR na STAT3 e sua rela??o com os diferentes perfis de LTCD8 de mem?ria. Resultados: Artigo de revis?o: Foram selecionados dezessete arquivos, sendo que destes dois foram exclu?dos. Artigo original: O VSR reduziu a express?o de STAT3 no lavado nasal de crian?as infectadas, porem aumentou a ativa??o de STAT3 via fosforila??o in vitro em c?lulas mononucleares infectadas. Conclus?o: A STAT3 ? parte importante na defini??o do perfil dos LTCD8 de mem?ria e sofre altera??es relevantes quando da infec??o pelo VSR, indicando uma nova possibilidade de alvo para imunoterapia.