N?veis da quimiocina RANTES na placenta, plasma materno e plasma do cord?o umbilical em gestantes normais e com s?ndrome de pr?-ecl?mpsia

• Main Author: Silveira, Ilson Dias da
• Other Authors: Costa, Bartira Erc?lia Pinheiro da
• Format: Others
• Language: Portuguese
• Published: Pontif?cia Universidade Cat?lica do Rio Grande do Sul 2015
• Subjects: MEDICINA; PLACENTA; PLASMA; CITOCINAS; PR?-ECL?MPSIA; GESTA??O; CORD?O UMBILICAL; MARCADORES BIOL?GICOS; CNPQ::CIENCIAS DA SAUDE::MEDICINA
• Online Access: http://tede2.pucrs.br/tede2/handle/tede/1706

Made available in DSpace on 2015-04-14T13:35:35Z (GMT). No. of bitstreams: 1 443353.pdf: 1896369 bytes, checksum: 19ecf303e539b93de8c9bda585ba0ebd (MD5) Previous issue date: 2012-08-28 === Objective: To estimate RANTES levels at maternal plasma, umbilical cord blood plasma and placenta in preeclampsia syndrome and normal pregnancy. Methods: A cross-sectional study was conducted to estimate RANTES levels in maternal plasma, placenta and cord blood plasma in preeclampsia syndrome (n=33) compared to a control group of women with normal pregnancy (n=36) attended at S?o Lucas Hospital/PUCRS, Porto Alegre, RS Brazil. Concentrations of RANTES were measured using a human RANTES ELISA assay. Results: Women with preeclampsia syndrome showed significantly higher RANTES levels in plasma and placenta in comparison to women with normal pregnancy (P<0.01). The cord blood plasma of infants born from women with preeclampsia syndrome had a significantly decreased RANTES levels compared to infants born from a normal pregnancy (P<0.01). The concentration of placental RANTES was positively correlated with RANTES in the maternal plasma (Pearson?s correlation r= 0.697; p<0.001), and negatively with the cord blood plasma (r= -0.818; p<0.001). A strong negative correlation was also demonstrated between maternal plasma and cord blood plasma RANTES (r= -0.751, p<0.001). Conclusion: This study shows a distinct inflammatory response between mother and new born in preeclampsia syndrome and normal pregnancy. In preeclampsia syndrome maternal plasma and placenta displayed increased pro-inflammatory RANTES cytokine concentration, however a reduced levels were detected in the umbilical cord plasma from women with preeclampsia syndrome, suggesting a fetal antiflammatory response. === Objetivo: O objetivo deste trabalho foi estimar os n?veis de RANTES na placenta e nos plasmas materno e do cord?o umbilical na s?ndrome de pr?-ecl?mpsia e gesta??o normal. M?todos: Estudo transversal e observacional estimou os n?veis de RANTES na placenta e nos plasmas materno e do cord?o umbilical na s?ndrome de pr?-ecl?mpsia (n=33) comparando com um grupo controle de gestantes normais (n=36) atendidas no Hospital S?o Lucas/PUCRS, Porto Alegre, RS, Brasil. As concentra??es de RANTES foram verificadas atrav?s do m?todo de enzima imuno ensaio (ELISA) espec?fico para humanos. Resultados: Mulheres com s?ndrome de pr?-ecl?mpsia apresentaram n?veis significativamente maiores de RANTES no plasma e placenta quando comparadas a gestantes normais (P<0,01). O plasma do cord?o umbilical de rec?m-nascidos de gestantes com pr?-ecl?mpsia mostraram n?veis de RANTES significativamente menores que o plasma do cord?o umbilical de rec?m-nascidos de gestantes normais (P<0,01). A concentra??o de RANTES placent?rio correlacionou-se positivamente com a concentra??o de RANTES no plasma materno (r= 0,697; P<0,001) e negativamente com o plasma do cord?o umbilical (r= - 0,818; P< 0,001). Forte correla??o negativa na concentra??o de RANTES foi demonstrada entre plasma materno e plasma do cord?o umbilical (r= - 0,751; P<0,001). Conclus?o: Este estudo mostra uma resposta inflamat?ria distinta entre a m?e e o rec?m-nascido em ambas as condi??es, gesta??o normal e s?ndrome de pr?-ecl?mpsia. A placenta e o plasma materno demonstraram uma concentra??o da citocina inflamat?ria RANTES aumentada na s?ndrome de pr?-ecl?mpsia, entretanto, no plasma do cord?o umbilical dos rec?m-nascidos dessas gestantes estava reduzida, sugerindo uma resposta fetal anti-inflamat?ria.