Propriedades oncolíticas do vírus da cinomose canina: expressão da proteína 8 (TNFAIP8) em células de carcinoma mamário: Juliana Albarracin Garcia. -

• Main Author: Garcia, Juliana Albarracin [UNESP]
• Other Authors: Universidade Estadual Paulista (UNESP)
• Format: Others
• Language: Portuguese
• Published: Universidade Estadual Paulista (UNESP) 2016
• Subjects: Cinomose; Cães; Mamas - Cancer; Oncologia veterinaria; Fenotipo; Marcadores biológicos de tumor; Fator de necrose de tumor; alpha-induced protein 8
• Online Access: http://hdl.handle.net/11449/138431; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/15-04-2016/000864249.pdf

Made available in DSpace on 2016-05-17T16:51:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-06. Added 1 bitstream(s) on 2016-05-17T16:54:50Z : No. of bitstreams: 1 000864249.pdf: 7320039 bytes, checksum: 72267d35491b03d5cd6737c361481e7c (MD5) === Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) === Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrossarcoma cell line were tested regarding to their susceptibility to CDV infection, apoptosis, mitochondrial membrane potential (MMP) and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication induced cytopathic effect and > 45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDV M gene expression were positively correlated in all cell lines. In addition, MMP was highly associated with increase in virus titres (p<0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy