Bioprospecção de fungos endofílicos Camarops sp., Periconia atropurpurea e Pseudofusicoccum stromaticum e avaliação epigenética de Phoma sp

• Main Author: Gubiani, Juliana Regina [UNESP]
• Other Authors: Universidade Estadual Paulista (UNESP)
• Format: Others
• Language: Portuguese
• Published: Universidade Estadual Paulista (UNESP) 2016
• Subjects: Fungos endofíticos; Plantas do deserto; Metabólitos; Epigenética; Desert plants
• Online Access: http://hdl.handle.net/11449/135922

Made available in DSpace on 2016-03-07T19:20:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-24. Added 1 bitstream(s) on 2016-03-07T19:24:01Z : No. of bitstreams: 1 000854407_20170724.pdf: 663761 bytes, checksum: 60eaa36c360bd21a72c549385424c3ff (MD5) Bitstreams deleted on 2017-07-24T11:34:16Z: 000854407_20170724.pdf,. Added 1 bitstream(s) on 2017-07-24T11:35:24Z : No. of bitstreams: 1 000854407.pdf: 8174488 bytes, checksum: 9e24d2d63d9e50b4f003bc5a41502cda (MD5) === Os fungos são considerados fontes promissoras de compostos bioativos com potencial aplicação na indústria alimentícia, agricultura e medicina, e de fato, diversos medicamentos utilizados na saúde pública são originárias de fungos. Estes são encontrados em vários ecossistemas, entre os quais podemos citar os que vivem no interior das espécies vegetais. Estes micro-organismos são denominados de endófitos e tem se revelado uma fonte promissora de metabólitos bioativos. Dentro deste contexto, este trabalho foi idealizado, objetivando a obtenção de substâncias potencialmente bioativas a partir do estudo químico e biológico dos extratos produzidos pelos fungos endofíticos Camarops sp., Periconia atropurpurea e Pseudofusicoccum stromaticum isolados de espécies vegetais do Cerrado e, o estudo epigenético de Phoma sp. isolado de espécie vegetal do Deserto de Sonora, utilizando no cultivo o modificador epigenético SAHA. Estes endófitos foram cultivados em escala reduzida em meios de cultivo líquidos e sólidos para obtenção dos extratos brutos, os quais foram submetidos a análises químicas (CCDC, HPLC e RMN de 1H) e biológicas (antifúngico, anticolinesterásico e citotóxico). Todos os extratos brutos apresentaram pelo menos uma atividade biológica, o que adicionado às análises químicas permitiram selecionar os meios de cultivo adequados para o crescimento em escala ampliada e, isolamento dos metabólitos secundários. Do cultivo de Camarops sp. no meio sólido de Milho isolou-se cinco substâncias, das quais quatro são inéditas 3-((1S,2S)-1,2-dihidroxibut-3-enil)-4-((E)-pent-1-enil)furan-2(5H)-ona (1), 3, 5, 9-trihidroxi presilfiperfolano (2), deacetilbotridial (3), ácido (E)-2,4-dimetilocta-2-enóico (4) e, ácido (E)-2,4-dimetilnon-2-enóico (5), os compostos 4 e 5 foram ativos na inibição da enzima acetilcolinesterase. Do extrato, obtido do meio líquido de Malte, de... === Fungi are considered promising sources of bioactive compounds with potential application in the food, agriculture and medicine industry, and in fact, many drugs used in public health originate from fungi. These are found in various ecosystems, among which we can mention those who live inside the plant species. These microorganisms are called endophytes and have proved to be a promising source of bioactive metabolites. Within this context, this work was carried out, aiming to obtain potentially bioactive substances from chemical and biological study of extracts produced by the endophytic fungi Camarops sp., Periconia atropurpurea and Pseudofusicoccum stromaticum isolated from plant species of the Cerrado and epigenetic studies of Phoma sp. isolated from plant species of the Sonoran Desert using in cultivation the epigenetic modifier SAHA. These endophytes were grown on small scale in liquid culture media and solid to obtain the crude extract. These were subjected to chemical analysis (TLC, HPLC and 1H NMR) and biological (antifungal, anticholinesterase and cytotoxic). All crude extracts showed at least one biological activity, which added the chemical analysis allowed to select the culture medium of appropriate for growth in large scale and isolation of secondary metabolites. The growing of Camarops sp. in solid medium of corn were isolated five compounds of which four are new 3-((1S,2S)-1,2-dihydroxybut-3-enyl)-4-((E)-pent-1-enyl)furan-2(5H)-one (1), 3, 5, 9-trihydroxy presilphiperfolane (2), deacetyl-botrydial (3), (E)-2, 4-dimetilocta-2-enoic acid (4), and (E)-2, 4-dimetilnon-2-enoic acid (5), compounds 4 and 5 were active in inhibiting the enzyme acetylcholinesterase. From the extract obtained from the liquid medium Malt, of P. atropurpurea were isolated the compounds 4,5-diethyl-3,4,5,6-tetrahydrobenzo[c][1,6]dioxecine-1,8-dione (6) and periconicina B (7). From the cultivation of P. stromaticum...