Avalia??o do perfil de sono/vigilia em um modelo cr?nico de parkinsonismo em ratos

• Main Author: Silva, Cinthya Montenegro de Vasconcelos
• Other Authors: 05189619400
• Language: Portuguese
• Published: PROGRAMA DE P?S-GRADUA??O EM PSICOBIOLOGIA 2017
• Subjects: CNPQ::CIENCIAS BIOLOGICAS: PSICOBIOLOGIA; Doen?a de Parkinson; Sintomas n?o-motores; Sistema de temporiza??o circadiano; Ritmos biol?gicos; Imunoistoqu?mica
• Online Access: https://repositorio.ufrn.br/jspui/handle/123456789/21583

Submitted by Automa??o e Estat?stica ([e-mail]) on 2017-01-03T19:56:08Z No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) === Approved for entry into archive by Arlan Eloi Leite Silva ([e-mail]) on 2017-01-05T22:10:42Z (GMT) No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) === Made available in DSpace on 2017-01-05T22:10:42Z (GMT). No. of bitstreams: 1 CinthyaMontenegroDeVasconcelosSilva_DISSERT.pdf: 1465896 bytes, checksum: 0fbac94f7a38a84105e55f2ee7340731 (MD5) Previous issue date: 2016-05-13 === A doen?a de Parkinson (DP) ? uma desordem neurodegenerativa e progressiva com espectro cl?nico variado. Al?m dos sintomas motores cl?ssicos tamb?m podem surgir complica??es n?o-motoras, destacando-se a? problemas cognitivos, psiqui?tricos e auton?micos. Evid?ncias demonstram que tais sintomas n?o-motores frequentemente precedem o aparecimento dos sinais motores e s?o extremamente relevantes, dado o impacto negativo que causam na qualidade de vida dos indiv?duos. Os sintomas n?o-motores podem apresentar m?ltiplas causas, dentre as quais uma poss?vel disfun??o do sistema circadiano. Dessa forma, diversos processos fisiol?gicos influenciados pelo sistema de temporiza??o circadiano (STC), como o ciclo sono/vig?lia podem se mostrar alterados em pacientes acometidos pela DP. O STC ? respons?vel pela gera??o e manuten??o dos ritmos circadianos, que s?o oscila??es end?genas manifestadas pelos seres vivos em diversos processos fisiol?gicos e comportamentais, com per?odo em torno de 24 horas. Assim, ? fundamental a compreens?o dos efeitos da progress?o do processo patog?nico da DP sobre o perfil circadiano do ciclo sono/vig?lia e tamb?m de componentes do STC, em particular no n?cleo supraquiasm?tico (NSQ), o principal marcapasso do sistema. No presente estudo, ratos wistar jovens (6 meses) e de meia-idade (10 meses) foram submetidos a um modelo animal cr?nico de DP com administra??o de reserpina durante 20 dias. Ao longo do tratamento foram realizadas an?lises comportamentais do sono, bem como a avalia??o motora dos animais. Ap?s o fim do tratamento, foram realizadas an?lises imunoistoqu?micas no NSQ dos animais. Nossos resultados mostraram que o tratamento cr?nico com reserpina promoveu comprometimento motor progressivo tanto nos animais jovens quanto nos de meia-idade. Al?m disso, as an?lises comportamentais revelaram perturba??es no ciclo sono/vig?lia dos animais tratados em compara??o aos indiv?duos controle, incluindo avan?o na fase de sono e aumento na fragmenta??o do sono. As an?lises imunistoqu?micas n?o permitiram observar efeitos significativos do tratamento com reserpina sobre a composi??o neuroqu?mica do NSQ, contudo novos estudos s?o necess?rios para a avalia??o neuroqu?mica e morfom?trica desse importante marcapasso circadiano na DP. === Parkinson?s disease (PD) is a neurodegenerative and progressive disorder with varied clinical spectrum. In addition to the classic motor symptoms may also emerge non-motor complications, highlighting cognitive, psychiatric and autonomic problems. Evidence shows that such non-motor symptoms often precede the onset of motor signs and are extremely relevant given the negative impact they have on the quality of life of the individuals. Non-motor symptoms may present multiple causes, among which a possible dysfunction of the circadian system. Therefore, many physiological processes influenced by the circadian timing system (CTS) as the sleep / wake cycle can show alterations in PD patients. In this study we sought to evaluate the profile of sleep/wake behavior in a chronic model of PD in rats compared with possible neurochemical changes in the suprachiasmatic nucleus(SCN), the main pacemaker of the CTS. To this end, young (6 months) and middle-age (10 months) wistar rats were subjected to a treatment with repeated administration of reserpine (0.1 mg / kg) for 20 days. During treatment sleep behavioral analysis were performed as well as the motor assessment of the individuals. After the end of treatment, immunohistochemical analyzes were performed in the SCN of the animals. Our results showed that chronic treatment with reserpine promoted progressive motor impairment both in young as in middle-age animals. It is noticed significant losses from the 12th day of treatment. Furthermore, the behavioral analyzes revealed disturbances in sleep / wake cycle of the treated animals compared to control subjects, including advanced sleep phase and increased sleep fragmentation. Such changes were observed from the 6th day of treatment, prior to the onset of motor symptoms. The immunohistochemical analysis not allowed to observe significant effects of treatment with reserpine on the neurochemical composition of the SCN. Thus, our data support the observation that non-motor symptoms precede the onset of motor symptoms in PD and are extremely important for early clinical diagnosis of the disease.