Epidemiologia molecular dos v?rus dengue e zika no estado do Rio Grande do Norte, no per?odo de junho de 2014 a maio de 2015

• Main Author: Monteiro, Joelma Dantas
• Other Authors: 01016476469
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2016
• Subjects: CNPQ::CIENCIAS BIOLOGICAS: BIOLOGIA PARASIT?RIA; Dengue; Zika; Epidemiologia; Rio Grande do Norte
• Online Access: http://repositorio.ufrn.br/handle/123456789/21368

Submitted by Automa??o e Estat?stica ([e-mail]) on 2016-09-06T20:09:33Z No. of bitstreams: 1 JoelmaDantasMonteiro_DISSERT.pdf: 1933748 bytes, checksum: 9df92e8b5cabf8720a41eeed7ddf9603 (MD5) === Approved for entry into archive by Arlan Eloi Leite Silva ([e-mail]) on 2016-09-06T22:54:25Z (GMT) No. of bitstreams: 1 JoelmaDantasMonteiro_DISSERT.pdf: 1933748 bytes, checksum: 9df92e8b5cabf8720a41eeed7ddf9603 (MD5) === Made available in DSpace on 2016-09-06T22:54:25Z (GMT). No. of bitstreams: 1 JoelmaDantasMonteiro_DISSERT.pdf: 1933748 bytes, checksum: 9df92e8b5cabf8720a41eeed7ddf9603 (MD5) Previous issue date: 2016-01-26 === A dengue e a febre da zika s?o doen?as causadas por v?rus RNA de fita simples, polaridade positiva, pertencentes ? fam?lia Flaviviridae, g?nero Flavivirus e s?o transmitidos ao homem atrav?s da picada de vetores artr?podes hemat?fagos do g?nero Aedes. O Estado do Rio Grande do Norte (RN) convive com o aumento do n?mero de casos da dengue h? mais de duas d?cadas, por?m, no que concerne ? febre da zika, at? os primeiros dias do m?s de maio deste ano, o Minist?rio da Sa?de n?o havia confirmado nenhum caso da doen?a no Brasil, tampouco no Rio Grande do Norte. Esse trabalho objetivou analisar o perfil epidemiol?gico dos v?rus Dengue e Zika no Estado do Rio Grande do Norte, no per?odo de junho de 2014 a maio de 2015. Foram estudadas 396 amostras provenientes de pacientes com casos suspeitos de dengue e/ou zika, das quais, 334 amostras foram analisadas para dengue atrav?s da RT-PCR, representando 8,08% (27/334) de positividade e 348 amostras foram estudadas para Zika por meio da qRT-PCR, onde o v?rus foi confirmado em 20,98% (73/348) dos casos. Os sorotipos DENV-1, DENV-2 e DENV-4 cocircularam no RN, com predomin?ncia do ?ltimo, detectado em 66,67% (18/27) dos casos positivos. Os munic?pios de Janda?ra, Natal e Ouro Branco foram os mais acometidos por dengue, com 18,52% (5/27), 22,22% (6/27) e 11,11% (3/27), respectivamente. No que se refere ao Zika, as cidades mais atingidas foram Guamar?, Natal, Nova Cruz e Parnamirim, com 13,70% (10/73), 30,14% (22/73), 9,59% (7/73) e 19,18% (14/73) de confirma??es, respectivamente. Neste estudo, o primeiro munic?pio a ter um caso confirmado de dengue foi Janda?ra. Cai?ara do Rio do Vento foi o primeiro a apresentar um caso de Zika, seguido de Galinhos. Junho foi o m?s mais representativo para dengue com 44,44% (12/27) dos casos confirmados, enquanto Mar?o foi o mais acometido por Zika com 23,29% (17/73). O g?nero masculino e o feminino tiveram praticamente a mesma propor??o de casos positivos para dengue, com 51,85% (14/27) e 48,15% (13/27), respectivamente, enquanto o Zika acometeu um maior n?mero de mulheres, representando 57,53% (42/73). As faixas et?rias mais acometidas por dengue foram a de 11-20 e 51-60 anos, cada uma com 18,52% (5/27). No que concerne ao v?rus Zika, a faixa et?ria de 0-10 anos foi a mais acometida com 19,18% (14/73) dos casos confirmados, seguidas das faixas de 31-40 e 41-50 anos, onde cada uma destas representou 16,44% (12/73). Em decorr?ncia da cocircula??o desses flaviv?rus no RN, ? fundamental compreender a preval?ncia e a din?mica de circula??o de ambos os v?rus, no intuito de estabelecer medidas para controlar futuros surtos e epidemias no Estado. Este trabalho representa o maior estudo sobre o Zika v?rus no Estado do RN. === The etiological agents of dengue fever and zika are single-stranded RNA viruses with positive polarity belonging to the Flaviviridae family, genus flavivirus and is transmitted to humans through the bite of blood-sucking arthropod vectors of the genus Aedes. The state of Rio Grande do Norte (RN) lives with dengue cases for over two decades. Regarding the zika fever, until the first day of May 2015, the Ministry of Health had not confirmed any cases of the disease in Brazil, either in RN, although already had clinical suspicion of infection caused by this virus. This study aimed to analyze the epidemiological profile of Dengue and Zika virus in the state of Rio Grande do Norte, in the period June 2014 to May 2015. A total of 334 blood samples from patients were analyzed for dengue virus (DENV) by RT-PCR technique where 8.08% were positive and 348 samples which were analyzed to detection Zika v?rus (ZIKV) by means of qRT-PCR, of which 20.98% were positive. It was found the movement of DENV serotypes-1, DENV-2 and DENV-4 in infants in the study period, with a predominance of this, detected in 66.67% (18/27) of positive cases. The municipalities of Natal, Janda?ra and Ouro Branco had a higher number of confirmed cases to the DENV, with 22.22% (6/27), 18.52% (5/27) and 11.11% (3/27), respectively. With regard to the ZIKV, the cities with highest numbers of confirmed cases were Natal, Parnamirim, Guamar? e Nova Cruz with 30.14% (22/73), 19.18% (14/73), 13.70% (10/73) and 9, 59% (7/73) respectively. The first town to have a confirmed case of DENV during the study period was Janda?ra. Cai?ara of Rio of Vento was the first to present a case ZIKV, followed by Galinhos. June was the most representative month in the number of confirmed cases of DENV with 44.44% (12/27), while March was the month with the highest number of confirmed cases of ZIKV with 23.29% (17/73). The male and females have about the same proportion of detected cases for DENV, with 51.85% (14/27) and 48.15% (13/27) respectively, while the ZIKV occurred in a larger number of women, representing 57.53% (42/73) of cases. The age groups most affected by dengue were 11-20 and 51-60 years, each with 18.52% (5/27) each one. Regarding the ZIKV, the age of 0-10 years was the most frequent with 19.18% (14/73) of the confirmed cases, followed by ages 31-40 and 41-50 years, where each of these represented 16 44% (12/73). The comparative analysis of the viral load between 5 days of fever zika performed by qRT-PCR showed a viral load higher on the 3rd day of the disease. Once proven the presence of these two flavivirus in RN, it is necessary to know the prevalence and circulation dynamics of each of the local population in order to establish control measures aimed at avoiding future outbreaks and epidemics in the state. This study represents the largest study on the virus Zika in the state of RN.