Valida??o de esp?cies de Centropomus (Centropomidae, Perciformes) do litoral do Rio Grande do Norte atrav?s de caracteriza??o citogen?tica e molecular

• Main Author: Borges, Amanda T?rres
• Other Authors: 56615043491
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2016
• Subjects: CNPQ::CIENCIAS BIOLOGICAS: SISTEM?TICA E EVOLU??O; Citogen?tica de peixes; DNAr 16S; Mapeamento cromoss?mico; Hibridiza??o in situ; Robalo
• Online Access: http://repositorio.ufrn.br/handle/123456789/20517

Submitted by Automa??o e Estat?stica ([e-mail]) on 2016-05-11T00:01:53Z No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) === Approved for entry into archive by Arlan Eloi Leite Silva ([e-mail]) on 2016-05-18T23:49:59Z (GMT) No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) === Made available in DSpace on 2016-05-18T23:49:59Z (GMT). No. of bitstreams: 1 AmandaTorresBorges_DISSERT.pdf: 1877687 bytes, checksum: b058a4ab09d1ebaaae5ddbe87c8c32b1 (MD5) Previous issue date: 2015-03-27 === A fam?lia Centropomidae ? composta por tr?s g?neros, Centropomus, Lates e Psammoperca. Centropomus ? o grupo mais diversificado, apresentando seis esp?cies com ocorr?ncia no Oceano Atl?ntico Ocidental, C. undecimalis (Bloch, 1792), C. poeyi Ch?vez, 1961, C. parallelus Poey, 1860, C. mexicanus Bocourt, 1868, C. pectinatus Poey, 1860 e C. ensiferus Poey, 1860. Algumas destas esp?cies s?o consideradas cr?pticas, por apresentarem caracter?sticas morfol?gicas pouco resolutivas para fins de identifica??o. Apesar do grande interesse como recurso natural e para cultivo, aspectos sobre sua diversidade e padr?es cariot?picos s?o pouco conhecidos. Neste trabalho classifica??es morfol?gicas e compara??es de sequ?ncias mitocondriais 16S foram utilizadas para identifica??o das esp?cies do g?nero Centropomus com ocorr?ncia no Rio Grande do Norte, nordeste do Brasil. Duas esp?cies foram identificadas, C. undecimalis e C. mexicanus, que tiveram seus aspectos cromoss?micos analisados atrav?s de m?todos citogen?ticos cl?ssicos (colora??o convencional, bandamento C, Ag-RONs), colora??o com fluorocromos AT- e GC-espec?ficos, bandas de replica??o pela incorpora??o do an?logo de base 5?BrdU ( 5-bromo-2?-deoxiuridina) e mapeamento cromoss?mico in situ de sequ?ncias (TTAGGG)n e dos genes RNAr 18S e 5S. Ambas as esp?cies apresentaram 2n=48 cromossomos acroc?ntricos, com s?tios ribossomais (Ag-RON/DNAr 18S/Mitramicina+) no segundo par cromoss?mico, em posi??o telom?rica no bra?o longo de C. mexicanus) e intersticial em C. undecimalis. O par organizador nucleolar (par 2) se mostra um marcador citotaxon?mico resolutivo para estas duas esp?cies. Os dados gerados revelam uma menor diversidade de esp?cies de Centropomus do que se acreditava, sugerindo uma maior aten??o na identifica??o taxon?mica das esp?cies, tendo em vista otimizar a??es de explora??o comercial, conserva??o biol?gica e cultivo. === The Centropomidae family consists of three genera, Centropomus, Lates and Psammoperca. Centropomus is the most diverse group, with six Centropomus species occur in the Western Atlantic Ocean C. poeyi Ch?vez, 1961, C. parallelus Poey, 1860, C. mexicanus Bocourt, 1868, C. pectinatus Poey, 1860 and C. ensiferus Poey, 1860. Some of these species are considered cryptic, because of its morphological traits showed low resolution for identification purposes. Despite showing great interest as a natural resource and fish culture, aspects of their diversity and karyotypic patterns are poorly understood. In this work morphological identification and comparison of mitochondrial 16S gene sequence were used to identify the species of the genus Centropomus occurring in Rio Grande do Norte, northeastern Brazil. Two sepecies were identified, C. undecimalis and C. mexicanus, which had the chromosomal aspects analyzed, through Classical cytogenetic method analyzes (conventional staining, C-banding, Ag-NORs), fluorochrome staining AT- and GC-specific, replication bands by incorporating of the base analog 5-Bromo-2?-deoxyuridine (5-BrdU), in situ chromosomal mapping of (TTAGGG)n sequences and in situ chromosome mapping 18S and 5S rRNA genes. Both species show 2n=48 acrocentric chromosomes, with ribosomal sites (Ag-NOR/18S rDNA/ Mitramycin+) in second chromosomal pair, in telomeric position on the long arm in C. mexicanus and interstitial in C. undecimalis. The nuclear organization pair (pair 2) shown a resolutive cytotaxonomic marker for these two species. The generated data reveal a lower species diversity than previously believed, suggesting that greater attention should be paid in taxonomic identification of the species, in view of optimize commercial actions exploitation, biological conservation and cultivation.