An?lise fitoqu?mica e avalia??o das atividades anti-inflamat?ria, antipe?onhenta e citot?xica de extratos aquosos de Aspidosperma pyrifolium e Ipomoea asarifolia

• Main Author: Lima, Maira Concei??o Jer?nimo de Souza
• Other Authors: 96728647449
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2016
• Subjects: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA; Aspidosperma pyrifolium; Ipomoea asarifolia; Tityus serrulatus; Bothrops jararaca; Atividade anti-inflamat?ria
• Online Access: http://repositorio.ufrn.br/handle/123456789/20105

Submitted by Automa??o e Estat?stica ([e-mail]) on 2016-03-22T19:30:53Z No. of bitstreams: 1 MairaConceicaoJeronimoDeSouzaLima_TESE.pdf: 6599799 bytes, checksum: 12ec2e74167618ab1f1b22a3284a60a5 (MD5) === Approved for entry into archive by Arlan Eloi Leite Silva ([e-mail]) on 2016-03-23T23:43:41Z (GMT) No. of bitstreams: 1 MairaConceicaoJeronimoDeSouzaLima_TESE.pdf: 6599799 bytes, checksum: 12ec2e74167618ab1f1b22a3284a60a5 (MD5) === Made available in DSpace on 2016-03-23T23:43:41Z (GMT). No. of bitstreams: 1 MairaConceicaoJeronimoDeSouzaLima_TESE.pdf: 6599799 bytes, checksum: 12ec2e74167618ab1f1b22a3284a60a5 (MD5) Previous issue date: 2015-04-17 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES === Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq === Envenenamentos causados por animais pe?onhentos s?o um grave problema de sa?de p?blica, enquadrando-se nesse cen?rio, principalmente, os escorpi?es e as serpentes. Tityus serrulatus ? considerado o escorpi?o mais venenoso da America do Sul, devido ? alta toxicidade do seu veneno, respons?vel por causar acidentes graves, principalmente em crian?as. A esp?cie Bothrops jararaca ? uma serpente que possui no seu veneno uma mistura complexa de enzimas, pept?deos e outras mol?culas. As toxinas do veneno de B. jararaca induzem respostas inflamat?rias locais e sist?micas. O tratamento de escolha para os casos graves de envenenamento ? a administra??o intravenosa do antiveneno especifico. Por?m, nem sempre esse tratamento est? acess?vel para os moradores de zonas rurais, que fazem uso de extratos de plantas medicinais. Nesse contexto, extratos aquosos, fra??es e compostos isolados de Aspidosperma pyrifolium (pereiro) e Ipomoea asarifolia (salsa), usadas na medicina popular, foram objeto de estudo deste trabalho para avaliar a atividade anti-inflamat?ria nos modelos de peritonite induzida por carragenina e peritonite induzida pelo veneno de T. serrulatus (VTs), e nos no modelo de edema local e infiltrado inflamat?rio induzido pelo veneno de B. jararaca, administrados pela via intravenosa. Os resultados dos ensaios de citotoxidade utilizando o ensaio MTT demonstraram que os extratos aquosos das esp?cies vegetais apresentaram baixa toxicidade para c?lulas provenientes de fibroblasto de embri?o de camundongo (3T3). A an?lise qu?mica dos extratos por Cromatografia L?quida de Alta Efici?ncia revelou a presen?a do flavon?ide rutina, na A. pyrifolium, e rutina, ?cido clorog?nico e ?cido caf?ico, na I. asarifolia. Quanto ? avalia??o farmacol?gica, os resultados demonstraram que o pr?-tratamento com extratos aquosos e fra??es reduziram a migra??o de leuc?citos totais para a cavidade abdominal no modelo de peritonite causada por carragenina e no modelo de peritonite induzida por veneno de T. serrulatus. Ainda, esses grupos apresentaram atividade antiedematog?nica, no modelo de edema local causado pelo veneno de B. jararaca, e reduziram o infiltrado inflamat?rio para o m?sculo. Os soros (antiaracn?dico e anti-botr?pico) espec?ficos para cada veneno atuaram inibindo a a??o inflamat?ria dos venenos e foram utilizados como controles. Os compostos identificados nos extratos, rutina, ?cido clorog?nico e ?cido caf?ico, tamb?m foram testados e, assim como os extratos das plantas, exibiram efeitos anti-inflamat?rios significativos, nas doses testadas. Dessa forma, esses resultados d?o evid?ncias, ?s plantas estudadas, de potencial atividade anti-inflamat?ria. Esse ? o primeiro estudo que avaliou os poss?veis efeitos terap?uticos de A. pyrifolium e I. asarifolia, mostrando o potencial terap?utico que essas esp?cies possuem. === Envenomation caused by venomous animals, mainly scorpions and snakes, are a serious matter of public health. Tityus serrulatus is considered the most venomous scorpion in South America because of the high level of toxicity of its venom. It is responsible for causing serious accidents, mainly with kids. The species Bothrops jararaca is a serpent that has in its venom a complex mixture of enzyme, peptides and other molecules. The toxins of the venom of B. jararaca induce local and systemic inflammatory responses. The treatment chosen to serious cases of envenomation is the intravenous administration of the specific antivenom. However, the treatment is not always accessible to those residents in rural areas, so that they use medicinal plant extracts as the treatment. In this context, aqueous extracts, fractions and isolated compounds of Aspidosperma pyrifolium (pereiro) and Ipomoea asarifolia (salsa, salsa-brava), used in popular medicine, were studied in this research to evaluate the anti-inflammatory activity in the peritonitis models induced by carrageenan and peritonitis induced by the venom of the T. serrulatus (VTs), and in the local oedema model and inflammatory infiltrate induced by the venom of the B. jararaca, administrated intravenously. The results of the assays of cytotoxicity, using the MTT, showed that the aqueous extracts from the plant species presented low toxicity to the cells that came from the fibroblast of the mouse embryo (3T3).The chemical analysis of the extracts by High Performance Liquid Chromatography revealed the presence of the rutin flavonoid, in A. pyrifoliu, and rutin, clorogenic acid and caffeic acid, in I. asarifolia. Concerning the pharmacological evaluation, the results showed that the pre-treatment using aqueous extracts and fractions reduced the total leukocyte migration to the abdominal cavity in the peritonitis model caused by the carrageenan and in the peritonitis model induced by the T. serulatus venom. Yet, these groups presented anti-oedematous activity, in the local oedema model caused by the venom of the B. jararaca, and reduced the inflammatory infiltrate to the muscle. The serum (anti-arachnid and anti-bothropic) specific to each venom acted inhibiting the inflammatory action of the venoms and were used as control. The compounds identified in the extracts were also tested and, similar to the plant extracts, showed meaningful anti-inflammatory effects, in the tested doses. Thus, these results are indicating the potential anti-inflammatory activity of the plants studied. This is the first research that evaluated the possible biological effects of the A. pyrifolium and I. asarifolia, showing the biological potential that these species have.