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Previous issue date: 2014-12-18 === A les?o perif?rica de c?lulas gigantes (LPCG) e a les?o central de c?lulas gigantes (LCCG) s?o les?es histologicamente semelhantes que acometem a regi?o de cabe?a e pesco?o. O estudo teve a finalidade de analisar a express?o imuno-histoqu?mica atrav?s dos marcadores GLUT-1, GLUT-3 e M-CSF em uma s?rie de casos de les?o perif?rica e central de c?lulas gigantes, na tentativa de estabelecer poss?veis associa??es e correla??es entre a express?o destas prote?nas nessas les?es, buscando uma melhor compreens?o do diferente comportamento biol?gico dessas entidades patol?gicas. A amostra foi constitu?da por 20 esp?cimes teciduais emblocados em parafina de LPCG, 20 de LCCG n?o agressivo e 20 de LCCG agressivo, oriundos do Servi?o de Anatomia Patol?gica da Disciplina de Patologia Oral do Departamento de Odontologia da UFRN. Em rela??o ao GLUT-1, verificou-se uma diferen?a estatisticamente significante (p< 0,05) na quantidade de c?lulas mononucleares imunomarcadas entre a les?o perif?rica (LP) e a les?o central n?o agressiva (LCNA) e entre a LP e a les?o central agressiva (LCA). Em rela??o ? intensidade da marca??o tamb?m foi verificado uma diferen?a estatisticamente significante tanto para as c?lulas mononucleares quanto para as c?lulas gigantes entre LP e LCNA e entre LP e LCA, nas c?lulas gigantes tamb?m ocorreu uma diferen?a estatisticamente significante entre a LCNA e a LCA. Em rela??o ao GLUT-3, foi encontrada uma diferen?a estatisticamente significante entre LP e LCA e entre LCNA e LCA na quantidade de c?lulas mononucleares imunomarcadas. No que concerne ? intensidade de marca??o para a referida prote?na foi verificado uma diferen?a estatisticamente significante nas c?lulas gigantes entre LP e LCA. Para o M-CSF foi observado apenas uma diferen?a estatisticamente significante na intensidade de marca??o nas c?lulas mononucleares entre LP e LCNA e entre LP e LCA. Com base nestes resultados, pode-se concluir a participa??o do GLUT-1, GLUT-3 e do M-CSF na patog?nese das les?es estudadas. Os transportadores de glicose estariam envolvidos no fornecimento de energia, para o metabolismo energ?tico das c?lulas e a prote?na osteoclastog?nica estaria envolvida no mecanismo de reabsor??o ?ssea encontrada nessas les?es. === The
peripheral
giant cell lesion
(
PG
CL
)
and
the
central
giant cell lesion
(
CGC
L)
are
lesions
histologically
similar
affecting the
head and neck
region
.
The study
aimed to
analyze the
immunohistochemical expression
of
markers GLUT
-
1
,
GLUT
-
3
and
M
-
CSF
in a series of
cases of
PGCL
and
CGCL
,
in trying to understand
the different
biological
behavior
of these
pathologies
.
The
sample consisted of
20
tissue
specimens
of
PGCL 20
central lesion
of
not
aggressive
giant cell
(
CLNAGC)
and 20
central lesi
on
of aggressive
giant cell
(
CLAGC),
coming from the
Pathology Unit
of
Oral Pathology
of
the Department of
Dentistry
of UFRN
.
W
as performed the s
emi
-
quantitative
and
qualitative analysis of
immunohistochemical expression
of the markers in
giant cells
and
m
ononuclear cells
.
In relation to the
GLUT
-
1, it was found
a statistically
significant
difference (p
<
0.05)
in the number of
mononuclear
cells
immunomarked
between the
PGCL
and
the
CLNAGC
and between
the
PGCL
and
CLAGC
.
Regarding the
intensity
of
staining w
as
also observed
a statistically
significant difference
both
at the
mononuclear cells
as in
giant cells
between
PL
and
CLNAGC
and between
PGCL
and
CLAGC
,
at the
giant cells
there was also a
statistically
significant difference
between
the
CLNAGC
and
CLAGC
.
In relation to
GLUT
-
3
,
was found
a statistically
significant
difference
between
PGCL
and
CLAGC
and
between
CLAGC
and
CLNAGC
in
amount
of
mononuclear cells
immunomarked
.
Regarding the intensity of labeling for such
protein was found a statistically
signifi
cant difference at
the giant cells between PL and
CLAGC
.
To
the
M
-
CSF was observed only a statistically
significant difference in
the
intensity
of labeling at
the
mononuclear cells between
PGCL
and
CLNAGC
and
between
PGCL
and
CLAGC
.
Based on these results,
we can conclude the participation
of GLUT
-
1, GLUT
-
3 and M
-
CSF in the pathogenesis of the lesions studied.
The bigger
immunostaining of these proteins in mononuclear cells show that these cells
perform
a
higher metabolic activity and osteoclastogenic, espe
cially in
CLAGC
. It was found that
the mononuclear cells were more related to the pathogenesis of the studied
lesions
than
properly
the giant
s
cell
s.
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