An?lise da imuno express?o das prote?nas IL-17, IL-23 E ROR?t na patogenia da doen?a periodontal

• Main Author: Aguiar J?nior, Jos? Nazareno Moreira de
• Other Authors: 82134731400
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2016
• Subjects: CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA: PATOLOGIA ORAL; Doen?as periodontais; Sistema imunol?gico; C?lulas T; C?lulas Th17; Citocinas
• Online Access: http://repositorio.ufrn.br/handle/123456789/19913

Submitted by Automa??o e Estat?stica ([e-mail]) on 2016-02-29T22:55:50Z No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) === Approved for entry into archive by Arlan Eloi Leite Silva ([e-mail]) on 2016-03-02T19:49:24Z (GMT) No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) === Made available in DSpace on 2016-03-02T19:49:24Z (GMT). No. of bitstreams: 1 JoseNazarenoMoreiraDeAguiarJunior_TESE.pdf: 2810253 bytes, checksum: d577f52edf024bf1bd64c021a5bed745 (MD5) Previous issue date: 2015-02-24 === A doen?a periodontal ? uma condi??o inflamat?ria cr?nica de car?ter infeccioso causada primariamente por bact?rias presentes em um biofilme dent?rio que interagem com o hospedeiro, determinando, assim, a natureza da doen?a resultante. Apesar de j? se conhecer muito sobre a patog?nese destas patologias, ainda n?o se sabe a composi??o exata do perfil de c?lulas T durante a fase ativa da doen?a (Th1, Th2 ou Th17). Este trabalho visou avaliar, atrav?s da express?o imuno-histoqu?mica, a presen?a dos marcadores (IL-17, IL-23 e ROR?t), envolvidos na resposta Th 17 em casos de gengiva clinicamente saud?veis (n=32), gengivite induzida pelo biofilme dental (n=30), periodontite cr?nica (n=32) e periodontite agressiva (n=25), objetivando analisar se a express?o e/ou distribui??o destas mol?culas em linf?citos e macr?fagos, presentes no infiltrado inflamat?rio dos tecidos periodontais, influencia na destrui??o tecidual observada nestas doen?as. Foi realizada a an?lise morfol?gica dos casos, onde avaliou-se a intensidade do infiltrado inflamat?rio em leve, moderado e intenso. Para cada caso, nas ?reas mais imunomarcadas, 5 campos foram escolhidos e analisados, tanto em rela??o a intensidade do infiltrado inflamat?rio quanto a quantidade de c?lulas imunomarcadas, baseando-se em escores predeterminados: escore 0 (aus?ncia de infiltrado inflamat?rio/imunomarca??o), escore 1 (o infiltrado/imunomarca??o abrangia menos de 25% da ?rea do campo), escore 2 (o infiltrado/imunomarca??o ocupava entre 25 e 50%) e escore 3 (infiltrado/imunomarca??o presente em mais de 50% da ?rea do campo). A partir disto, gerouse uma mediana que representava cada caso. A intensidade do infiltrado inflamat?rio foi correlacionada com a progress?o da doen?a, se mostrando crescente da gengiva clinicamente saud?vel at? a periodontite agressiva (p<0,001). Detectou-se a presen?a da IL-17, IL-23 e do ROR?t na maioria dos casos avaliados e a quantidade de c?lulas imunomarcadas foi correlacionada tanto com a intensidade do infiltrado inflamat?rio (P<0,001) quanto com os par?metros cl?nicos analisados (P<0,001), apresentando uma correla??o positiva, predominantemente moderada. A periodontite agressiva apresentou um maior percentual de imunomarca??o em rela??o ?s outras condi??es cl?nicas avaliadas, para todos os marcadores, sugerindo uma poss?vel associa??o destes marcadores com a progress?o desta doen?a, onde quanto maior a perda de suporte periodontal, maior a quantidade do infiltrado inflamat?rio e maior n?mero de c?lulas imunomarcadas. === Periodontal disease is a chronic inflammatory condition primarily caused by bacteria in dental biofilm, which interact with the host, thus determining the nature of the resulting disease. Despite the wide knowledge about the pathogenesis of these diseases, the exact composition of the T cell profile during the active phase of the disease (Th1, Th2 or Th17) remains unknown. This study aimed to evaluate by immunohistochemical expression, the presence of the markers (IL-17, IL-23 and ROR?t), involved in Th17 response in clinically healthy gingiva cases (n = 32), biofilm-induced gingivitis (n = 30), chronic periodontitis (n = 32) and aggressive periodontitis (n = 25), in order to analyze if the expression and/or distribution of these molecules in lymphocytes and macrophages, present in the inflammatory infiltrate of periodontal tissue, influences the tissue destruction observed in these diseases. The morphological analysis of cases was performed which assessed the intensity of the inflammatory infiltrate in mild, moderate and intense. For each case, in the area with the most representative immunostaining, 5 fields were chosen and analyzed, both for the intensity of the inflammatory infiltrate as for the quantity of immunostained cells, based on predetermined scores: score 0 (absence of inflammatory infiltrate/immunostaining), score 1 (the infiltrate/immunostaining covered less than 25% of the field area), score 2 (the infiltrate/immunostaining occupied between 25 and 50%) and score 3 (infiltrate/immunostaining present in over 50% of the field area). From this, a median was generated representing each case. The intensity of the inflammatory infiltrate correlated with the disease progression, in other words, it was crescent from clinically healthy gingiva to aggressive periodontitis (P <0.001). It was detected the presence of IL-17, IL-23 and ROR?t in most of the evaluated cases and the number of immunostained cells correlated with the intensity of the inflammatory infiltrate (P <0.001) and with the clinical parameters analyzed (P <0.001), showing a positive correlation, mainly moderate. Aggressive periodontitis showed a higher percentage of immunostaining for all markers in relation to other clinical conditions assessed, suggesting a possible association of these markers with the progression of this disease, in which the higher the loss of periodontal support, the greater the amount of inflammatory infiltrate and larger the number of immunostained cells.