Desenvolvimento de emuls?es o/a contendo extrato glic?lico de a?ai e avalia??o da atividade fotoprotetora

• Main Author: Daher, Cl?udia Cecilio
• Other Authors: 12835182870
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2015
• Subjects: Estabilidade F?sica; Euterpe oleracea; Fotoprotetor; Fator de Prote??o Solar UVA
• Online Access: http://repositorio.ufrn.br/handle/123456789/19364

Submitted by Automa??o e Estat?stica ([e-mail]) on 2015-11-12T14:44:33Z No. of bitstreams: 1 ClaudiaCecilioDaher_DISSERT.pdf: 1582932 bytes, checksum: a5b87b42e15b284fd48200411e8eea44 (MD5) === Approved for entry into archive by Elisangela Moura ([e-mail]) on 2015-11-16T12:26:17Z (GMT) No. of bitstreams: 1 ClaudiaCecilioDaher_DISSERT.pdf: 1582932 bytes, checksum: a5b87b42e15b284fd48200411e8eea44 (MD5) === Made available in DSpace on 2015-11-16T12:26:17Z (GMT). No. of bitstreams: 1 ClaudiaCecilioDaher_DISSERT.pdf: 1582932 bytes, checksum: a5b87b42e15b284fd48200411e8eea44 (MD5) Previous issue date: 2014-03-27 === Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq === O uso de protetor solar ? uma das medidas de fotoprote??o mais utilizada pela popula??o, assim esses produtos devem oferecer prote??o de amplo espectro contra as radia??es UVA e UVB. Subst?ncias vegetais t?m sido recentemente consideradas um recurso para ser utilizadas em formula??es fotoprotetoras devido a sua absor??o no espectro UV e propriedades antioxidantes. A Euterpe oleraceae Mart., conhecida popularmente como a?a?, cont?m em sua composi??o qu?mica, compostos polifen?licos como as antocianinas e flavonoides, aos quais ? atribu?da a propriedade antioxidante. Esta pesquisa teve como objetivos desenvolver e avaliar a estabilidade de emuls?es O/A contendo extrato glic?lico de a?a? e avaliar a efic?cia fotoprotetora. A seguran?a do extrato foi determinada por meio de testes in vitro de citotoxicidade e fototoxicidade. Emuls?es contendo extrato glic?lico de a?a? foram formuladas utilizando diferentes tipos e concentra??es de emulsionantes polim?ricos (Acrylates/C 10-30 Alkyl Acrylate Crosspolymer e Sodium Polyacrylate). A influ?ncia de dois modificadores reol?gicos (Carbomer e Polyacrylamide (and) C 13-14 Isoparaffin (and) Laureth-7) forma estudados com o objetivo de melhor a estabilidade. A estabilidade f?sica foi avaliada por meio de testes preliminares e acelerados. A an?lise macrosc?pica, determina??o dos valores de pH e condutividade el?trica e o comportamento reol?gico foram determinados em diferentes tempos. A determina??o do Fator de Prote??o Solar (FPS) in vivo foi realizado pelo m?todo COLIPA 2006, e o Fator de Prote??o UVA (FPUVA), comprimento de onda cr?tico e raz?o FPS/FPUVA, foram realizados de acordo com o m?todo in vitro COLIPA 2011. O extrato em estudo n?o apresentou-se citot?xico e fotot?xico. A emuls?o est?vel contendo 5% de extrato glic?lico de a?a? e 1,0% de Sodium Polyacrylate apresentou carater?sticas pseudopl?tica e tixotr?pica. A emuls?o contendo o extrato de a?a? obteve FPS 25,3 e FPUVA 14,97. A adi??o do extrato glic?lico de a?a? n?o proporcionou aumentou siginificante no FPS e o FPUVA. Esta emuls?o apresentou 1,69 de raz?o FPS/FPUVA e comprimento de onda cr?tico em 378 nm, podendo ent?o ser considerada um fotoprotetor de amplo espectro, com prote??o contra UVA e UVB. === Sunscreen use is the most common photoprotection alternative used by the population, and so these products should offer improved protection with broad - spectrum, UVA and UVB protection . Vegetal substances have recently been considered as resources for sunscreen formulations due to their UV spectrum absorption and antioxidant properties. The Euterpe oleracea Mart., popularly known as a?ai, in its che mical composition contain polyphenols compounds, such as anthocyanins and flavonoids , to which antioxidant properties have been attributed . The aim of this work was to develop O/W sunscreens emulsions con taining a?ai glycolic extract ( AGE) and to evaluate both their physical stability , safety and photoprotective efficacy. The safety of the extract was evaluated by in vitro phototoxicity and cytotoxicity tests. Emulsions containing AGE and sunscreens were formulated using different types and concentrations o f polymeric surfactant (Acrylates/C 10 - 30 Alkyl Acrylate Crosspolymer and Sodium Polyacrylate). The influence of two rheology modifiers (Polyacrylamide (and) C13 - 14/Isoparaffin (and) Laureth - 7 and Carbomer) on the stability was also investigated. Physical stability was evaluated by preliminary and accelerated studies. The macroscopic analyses, pH value and electrical conductivity determinations and rheological behavior were evaluated at different time intervals . The in vivo Sun Protect Factor ( SPF ) was determined according to the International Sun Protection Factor Test Method ? 2006 and UVA Protection Factor (FPUVA), wavelength critical and reason SPF/FPUVA were performed according to the method Colipa 2011. The extract did not present cytotoxic ity and phototoxic ity . The stable emulsion containing 5% glycolic extract of a?ai and 1.0% of sodium poliyacrylate showed pseudoplastic and thixotropic behavior . The sunscreen emulsion containing a?ai glycolic extract showed a SPF 25.3 and PF - UVA = 14.97. Whe n a?ai glycolic extract was added in the emulsion sunscreen, no significant increase in the in vivo SPF and FPUVA values were observed. This emulsion showed 1.69 of the SPF/PF - UVA ratio and a critical wavelength value of 378 nm, so may therefore be conside red a sunscreen with UVA and UVB protection.