Superf?cies de tit?nio modificadas termoquimicamente por plasma : avalia??o da resposta biol?gica

• Main Author: Aires, Michelle de Medeiros
• Other Authors: CPF:09621199468
• Format: Others
• Language: Portuguese
• Published: Universidade Federal do Rio Grande do Norte 2014
• Subjects: Seletividade celular; Biocompatibilidade; Tratamento por plasma; Biofilme; Staphylococcus epidermidis; Pseudomonas aeruginosa; Cellular selectivity; Biocompatibility; Plasma treatment; Biofilm; Pseudomonas aeruginos; CNPQ::CIENCIAS DA SAUDE
• Online Access: http://repositorio.ufrn.br:8080/jspui/handle/123456789/13351

Made available in DSpace on 2014-12-17T14:13:53Z (GMT). No. of bitstreams: 1 MichelleMA_DISSERT.pdf: 2721160 bytes, checksum: a6cf87e66222fcde35f5dcad4d548b9d (MD5) Previous issue date: 2011-08-05 === This laboratory study involves the participation of a group with professionals from different areas that had contributed to the construction of a multidisciplinary knowledge, about biological response of titanium surfaces modified through thermochemical treatment by plasma. Thus, the crystalline phase was previously characterized in relation to the topography, roughness, molhability and nitrogen concentration in the samples surface. It s indispensable that materials implanted can influence in a good cellular response as well as promotes a bacteria action. Surfaces modified by plasma were exposed to different cultures such as: cellular (human osteoblastic) and bacteria (Staphylococcus epidermidis ATCC35984 and Pseudomonas aeruginosa ATCC 27853) in order to evaluate the biological response. It was evaluated the adhesion, proliferation, morphology and cellular preference of human ostheoblastic cells (HOST), as well as the formation of a biofilm and bacteria proliferation. It was still analyzed the bacteria selectivity ability in relation to the surfaces. The software Image Pro Plus was used to the counting of cells and bacteria adhered to the surface of disks. The results were submitted to the variance analysis (ANOVA), and then, by the Kruskal-Wallis test, using GraphPad Instat ? software, version 3.5 to Windows. The nitrided samples in spite of show a higher roughness and molhability showed a smaller bacteria growing and higher cellular proliferation, when compared to non treated samples, indicating that the treated material present a high efficiency to biomedical implants === Este estudo laboratorial envolveu a participa??o de um grupo de profissionais de ?reas distintas, as quais contribu?ram na constru??o de um conhecimento multidisciplinar, acerca da resposta biol?gica de superf?cies de tit?nio modificadas por tratamento termoqu?mico a plasma. Para tanto, uma avalia??o da an?lise de fase cristalina, topografia, rugosidade, molhabilidade e concentra??o de nitrog?nio nas superf?cies das amostras foram previamente determinadas. ? imprescind?vel que materiais implant?veis influenciem uma boa resposta celular como tamb?m proporcionem uma a??o bacteriost?tica. Com o intuito de avaliar a resposta biol?gica, superf?cies modificadas por plasma foram expostas as seguintes culturas: celular (osteoblasto humano) e bacteriano (Staphylococcus epidermidis ATCC35984 e Pseudomonas aeruginosa ATCC 27853). Avaliou-se a ades?o, prolifera??o, morfologia e prefer?ncia de c?lulas osteobl?sticas humanas (HOST) bem como a forma??o de biofilme e prolifera??o bacteriana. Analisou-se, ainda, a capacidade de seletividade de superf?cie pelas bact?rias. O software Image Pro Plus foi utilizado para contagem das c?lulas e bact?rias aderidas ? superf?cie dos discos. Os resultados foram submetidos ? an?lise de vari?ncia (ANOVA), seguido pelo teste de Kruskal-Wallis, utilizando o GraphPad Instat ?, vers?o 3.5 para Windows. As amostras nitretadas apesar de apresentarem uma maior rugosidade e molhabilidade, quando comparadas com as amostras n?o tratadas, tiveram um menor crescimento bacteriano e uma maior prolifera??o celular. Indicando que o material tratado possui uma alta efici?ncia para implantes biom?dicos