Potencial antibacteriano de Agaricomycetes coletados no Nordeste do Brasil

• Main Author: SANTANA, Valéria Ferreira da Silva Costa
• Other Authors: http://lattes.cnpq.br/8535172322991148
• Language: Portuguese
• Published: Universidade Federal de Pernambuco 2018
• Subjects: Agaricus (cogumelo); Compostos bioativos; Bactérias
• Online Access: https://repositorio.ufpe.br/handle/123456789/25283

Submitted by Fernanda Rodrigues de Lima ([e-mail]) on 2018-07-26T19:43:46Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) DISSERTAÇÃO Valeria Ferreira da Silva Costa Santana.pdf: 752817 bytes, checksum: 775e0249c699e222cc8e47f7e2873e1e (MD5) === Approved for entry into archive by Alice Araujo ([e-mail]) on 2018-07-30T21:55:02Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) DISSERTAÇÃO Valeria Ferreira da Silva Costa Santana.pdf: 752817 bytes, checksum: 775e0249c699e222cc8e47f7e2873e1e (MD5) === Made available in DSpace on 2018-07-30T21:55:02Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) DISSERTAÇÃO Valeria Ferreira da Silva Costa Santana.pdf: 752817 bytes, checksum: 775e0249c699e222cc8e47f7e2873e1e (MD5) Previous issue date: 2015-02-26 === FACEPE === Agaricomycetes coletados em várias partes do mundo apresentam substâncias bioativas com atividade antialérgica, antitumoral, antiviral, antioxidante, imunomoduladora, antiteratogênica, hipoglicêmica, antiparasitária, antimicrobiana e imunoprotetora. Além disso, essas substâncias também ajudam o organismo a prevenir o diabetes e a fortalecer o sistema imunológico. Entretanto, poucos são os trabalhos com material coletado no Brasil. Assim, o presente estudo tem por objetivo incrementar o conhecimento sobre o potencial antibacteriano dos Agaricomycetes no país por meio de pesquisa com material coletado no Nordeste do Brasil. Para isso, foi realizado um inventário das espécies depositadas na Micoteca URM da UFPE. Às 32 culturas encontradas, foram acrescidos ao estudo uma cultura ainda a ser depositada e extratos de 11 basidiomas frescos. Dez culturas depositadas na Micoteca URM não puderam ser reativadas. As 23 culturas foram repicadas em meio sólido de extrato de Malte. Das culturas e dos basidiomas, foram obtidos extratos com acetato de etila, etanol e metanol, os quais foram solubilizados na concentração de (1:10 v/v). Os testes preliminares com os 34 materiais (23 culturas e 11 basidiomas frescos) foram realizados contra 11 linhagens de Staphyllococcus aureus, das quais sete resistentes a oxacilina (ORSA), a partir da metodologia de bloco de gelose. Quinze apresentaram atividade antibacteriana, com inibição entre 9–24 mm e concentração mínima inibitória (CMI) de 0,18–147 μg/mL, dentre os quais Fomitopsis cupreorosea (URM 6830), Ganoderma multiplicatum (URM 6975), G. parvulum (URM 2948), Ganoderma sp. (extrato de basidioma fresco), Grammothele lineata (URM 6827), Rigidoporus lineatus (URM 6828), R. microporus (URM 6878), Stereum ostrea (URM 6973) e Stereum ostrea (extrato de basidioma fresco) apresentaram forte inibição contra todas as linhagens de S. aureus testadas. === Agaricomycetes collected in several parts of the world have bioactives substances with anti-allergic, antifungal, anti-inflammatory, antiparasitic, antiviral, antioxidant, cytotoxic, immunomodulatory, antiatherogenic, hepatoprotective, hypoglycemic, antibacterial e imunoprotective. Besides, these substances help the organism to prevent diabetes and to strength the immune system. However, few are the studies with material collected in Brazil. Thus, the current study aimed to improve the knowledge about the antibacterial potential of Agaricomycetes in the country by researching material collected in Northeast Brazil. For this, the inventory of the species deposited in Micoteca URM of UFPE was done and 32 cultures were found. To these, one culture to be deposited and 11 extracts from fresh basidiomata were added to the study. Ten cultures could not be reactivated. The 23 cultures were transferred to solid medium of malt extract. Extracts of the basidiomata and the cultures were obtained with ethyl acetate, ethanol e methanol, which were solubilized at a concentration (1:10 v/v). The preliminary tests with the 34 materials (23 cultures and 11 extracts of fresh basidiomata) were performed against 11 strains of Staphyllococcus aureus, seven of which resistant to oxacilin (ORSA), using block agar. Fifteen showed antibacterial activity, with inhibition between 9–24mm and minimum inhibitory concentration (MIC) from 0.18–147 μg/mL, among which Fomitopsis cupreorosea (URM 6830), Ganoderma multiplicatum (URM 6975), G. parvulum (URM 2948), Gandoerma sp. (extract of fresh basidiomata), Grammothele lineata (URM 6827), Rigidoporus lineatus (URM 6828), R. microporus (URM 6878), Stereum ostrea (URM 6973) and Stereum ostrea (extracs of fresh basidiomata) showed strong inhibition against all strains of S. aureus.