Caracterização de cepas de Pseudomonas spp isoladas de efluente hospitalar não tratado : resistência a beta-lactâmicos e presença de integrons

• Main Author: Spindler, Aline
• Other Authors: Corção, Gertrudes
• Format: Others
• Language: Portuguese
• Published: 2011
• Subjects: Pseudomonas; Efluentes hospitalares : Porto Alegre (RS); Beta-Lactamas; Integrons; Resistência bacteriana
• Online Access: http://hdl.handle.net/10183/29010

As bactérias contendo este elemento de mobilidade genética podem contribuir para a disseminação de determinantes de resistência, bem como servir de reservatório em potencial para estes genes. === Pseudomonas genus, widely distributed in the environment, is often found associated to determinants of beta-lactams resistance and presence of integrons. The present study was conducted to investigate the resistance profile of Pseudomonas spp strains isolated from untreated hospital effluents, likewise beta-lactamase genes and integrons. Untreated hospital effluents were collected from four hospitals in Porto Alegre, RS. Non-fermenter bacteria were isolated; the identification was confirmed by 16S rRNA PCR. Susceptibility testing was determined by the disk-diffusion method using 11 different betalactams antimicrobials. The beta-lactamase genes blaIMP, blaVIM, blaSPM-1, blaOXA-23-like, blaOXA-24-like, blaOXA-51-like and blaGES-like genes and the integrons genes intI1, intI2 and intI3 were determined by PCR using specific primers. One hundred and twenty-eight isolates were recovered; the most common species was P. pseudoalcaligenes. The resistance level found was considered high, 62 (50%) isolates were multi-resistants. No isolate carrying the beta-lactamase genes tested was found among the strains. Of 68 isolates considered positives for integrons, 52 were identified as carrying the class 1 integron gene, intI1. No isolate carrying class 1 or class 2 integrons was found among the strains. Untreated hospital effluents could be a source of environmental contamination due to discharge of antimicrobial resistant bacteria, in the case of the present study, also integron positives. Bacteria carrying this genetic mobile element can contribute for the dissemination of resistance determinants and also act like a potential reservoir for many types of resistance genes.