Isolamento e Identifica??o de Bact?rias Potencialmente Patog?nicas a partir de Bivalves no Arquip?lago de Santana Maca?, RJ.

Made available in DSpace on 2016-04-28T20:17:29Z (GMT). No. of bitstreams: 1 2008 - Marcelo Santos de Oliva.pdf: 3554572 bytes, checksum: 0fae31785d5eaf0f107b4791b1e03628 (MD5) Previous issue date: 2008-11-13 === Mussels are bivalve mollusks that developed a filtrating system enabling them to upta...

Full description

Bibliographic Details
Main Author: Oliva, Marcelo Santos de
Other Authors: Souza, Miliane Moreira Soares de
Format: Others
Language:Portuguese
Published: Universidade Federal Rural do Rio de Janeiro 2016
Subjects:
Online Access:https://tede.ufrrj.br/jspui/handle/tede/879
Description
Summary:Made available in DSpace on 2016-04-28T20:17:29Z (GMT). No. of bitstreams: 1 2008 - Marcelo Santos de Oliva.pdf: 3554572 bytes, checksum: 0fae31785d5eaf0f107b4791b1e03628 (MD5) Previous issue date: 2008-11-13 === Mussels are bivalve mollusks that developed a filtrating system enabling them to uptake nutrients from water. This is a not selective mechanism, so mussels microbiological analysis shows up the aquatic environment quality. Therefore, the present work aimed to isolate and identify bacterial microbiota from bivalve mollusks incrustated into the rocky coast of Arquip?lago de Santana, Maca?, RJ. The antimicrobial resistance pattern of prevalent microorganisms isolated was also analyzed. The surrounding water microbiological quality was also evaluated in order to detect contamination source from fishing and subaquatic activities in the studied region. From the bacteriological analysis it was obtained a total of 51 isolates of Vibrio spp., being V. damsela (n=15) the prevalent specie, followed by V. harveyi (n=13) and V. alginolyticus (n=07). It was also obtained a total of 20 isolates of Enterobacteriaceae species, being Escherichia coli (n=06) the prevalent one, followed by Proteus vulgaris (n=04). Vibrio spp. isolates presented 100% of sensitivity to tested antimicrobials, except for ampicillin with no detected sensitivity corroborating to literature. For enterobacteria, it was detected a high percentile of sensitivity to all testes antimicrobials. In the six samples of ocean water analyzed it was not possible to detect total or fecal coliforms. The low percentile of isolated microorganisms from mussels at Arquip?lago de Santana can be justified for its location at the open sea, far away from the coast and influenced by sea currents, in a environment not yet altered by human action. === Os mexilh?es s?o moluscos bivalves filtradores que se alimentam de microrganismos captados pela corrente de ?gua e n?o apresentam capacidade seletiva de filtra??o do seu alimento, consequentemente, a an?lise dos mexilh?es reflete a qualidade microbiol?gica do habitat aqu?tico. Desse modo, o presente trabalho objetivou executar o isolamento e identifica??o da microbiota bacteriana a partir de moluscos bivalves incrustados em cost?es rochosos no Arquip?lago de Santana, Maca?- RJ, bem como analisar o perfil de resist?ncia dos microrganismos prevalentes. Tamb?m se buscou avaliar a qualidade da ?gua de modo a detectar poss?veis contamina??es decorrentes das atividades pesqueiras e subaqu?ticas nesta regi?o. A partir das an?lises bacteriol?gicas foi obtido um total de 51 col?nias de Vibrio spp. com preval?ncia da esp?cie V. damsela (n=15), seguida de V. harveyi (n=13) e V. alginolyticus (n=07) e um total de 20 col?nias de enterobact?rias com preval?ncia de Escherichia coli (n=06) seguida de Proteus vulgaris (n=04). Os isolados de Vibrio spp. apresentaram 100% de sensibilidade aos antimicrobianos testados, com exce??o da ampicilina, para a qual n?o foi detectada qualquer sensibilidade a semelhan?a de outros relatos na literatura. Nos isolados de enterobact?rias avaliados, foram detectados elevados percentuais de sensibilidade aos antimicrobianos testados. No total de seis amostras de ?gua do mar analisadas, n?o foi detectada a presen?a de coliformes totais e termotolerantes. O baixo percentual de microrganismos isolados de mexilh?es no Arquip?lago de Santana pode ser justificado por ser um local de mar aberto, afastado da costa e sobre a influ?ncia de correntes mar?timas, e ainda pouco impactado pela a??o humana.