Avalia??o da virul?ncia in vitro de Candida spp (BERKHOUT,1923) isoladas de orofaringe de Struthio camelus (LINNAEUS, 1758).

• Main Author: Bath, Felipe Vict?rio de Castro
• Other Authors: Baroni, Francisco de Assis
• Format: Others
• Language: Portuguese
• Published: Universidade Federal Rural do Rio de Janeiro 2016
• Subjects: Candida; avestruzes; virul?ncia.; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Online Access: https://tede.ufrrj.br/jspui/handle/tede/878

Made available in DSpace on 2016-04-28T20:17:29Z (GMT). No. of bitstreams: 1 2009 - Felipe Victorio de Castro Bath.pdf: 899860 bytes, checksum: 4fd602f8c11878cd5b06034ba2ab2b10 (MD5) Previous issue date: 2009-03-26 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior === The objective of this work was checked Candida's percentage spp in oropharynx of ostriches (Struthio camelus) and he valued in vitro the virulence of the isolated strains. The motivation for this research was due to the fact of the ostrich be exotic sorts and for the ignorance of the potential microrganisms that this one has. The animals were divided in four groups, totalizing 80 samples. The research of the presence of the agent in swabs originating from the birds was done in the Laboratory of Patogenic Yeasts and You set, Depto of Microbiology and Imunology Veterinary, Institute of Veterinary, UFRRJ, for processing of same, with sowings in environment of cultivation on Sabouraud dextrose agar. The confirmation of isolations were carried out through tests of macromorphology, micromorphology, realization of auxanogram, zimogram and complementary proofs. Of the total of samples of the oropharynx, 32,5 % obtained growth with yeasts, only or accompanied by moulds. The growth of C. albicans corresponded to 65,38 % of the total of isolates. The most of the isolated ones were strongly positive for production of protease and approximately also they showed half of these positive samples for the production of phospholipases what suggests some degree of virulence of these strains. === O objetivo deste trabalho foi verificar o percentual de Candida spp em orofaringe de avestruzes (Struthio camelus) e avaliar a virul?ncia in vitro das esp?cies isoladas. A motiva??o para esta pesquisa deveu-se ao fato do avestruz ser uma esp?cie ex?tica e pelo n?o conhecimento do potencial de microrganismos que esta possui. Os animais foram divididos em quatro grupos, totalizando 80 amostras. A pesquisa da presen?a do agente nos swabs oriundos das aves foi feita no Lab. de Leveduras. Patog?nicas e Ambientais, Depto de Microbiologia e Imunologia Veterin?ria, IV, UFRRJ, por processamento dos mesmos, com semeadura em meio de cultivo ?gar Sabouraud dextrose. As confirma??es de isolamento foram realizadas por meio de testes de macromorfologia, micromorfologia, realiza??o de auxanograma, zimograma e provas complementares. Do total de amostras processadas da orofaringe, 32,5% obtiveram crescimento leveduriforme, ?nico ou acompanhado de fungos filamentosos. O crescimento de C. albicans correspondeu a 65,38% do total de isolados leveduriformes. A maioria dos isolados foi fortemente positiva para produ??o de protease e aproximadamente metade destas amostras tamb?m mostraram-se positivas para a produ??o de fosfolipases o que sugere a alta virul?ncia destas amostras. Palavras chave: Candida, avestruzes, virul?ncia.