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2009 - Felipe Victorio de Castro Bath.pdf: 899860 bytes, checksum: 4fd602f8c11878cd5b06034ba2ab2b10 (MD5)
Previous issue date: 2009-03-26 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior === The objective of this work was checked Candida's percentage spp in oropharynx of ostriches
(Struthio camelus) and he valued in vitro the virulence of the isolated strains. The motivation for
this research was due to the fact of the ostrich be exotic sorts and for the ignorance of the
potential microrganisms that this one has. The animals were divided in four groups, totalizing 80
samples. The research of the presence of the agent in swabs originating from the birds was done
in the Laboratory of Patogenic Yeasts and You set, Depto of Microbiology and Imunology
Veterinary, Institute of Veterinary, UFRRJ, for processing of same, with sowings in environment
of cultivation on Sabouraud dextrose agar. The confirmation of isolations were carried out
through tests of macromorphology, micromorphology, realization of auxanogram, zimogram and
complementary proofs. Of the total of samples of the oropharynx, 32,5 % obtained growth with
yeasts, only or accompanied by moulds. The growth of C. albicans corresponded to 65,38 % of
the total of isolates. The most of the isolated ones were strongly positive for production of
protease and approximately also they showed half of these positive samples for the production of
phospholipases what suggests some degree of virulence of these strains. === O objetivo deste trabalho foi verificar o percentual de Candida spp em orofaringe de avestruzes
(Struthio camelus) e avaliar a virul?ncia in vitro das esp?cies isoladas. A motiva??o para esta
pesquisa deveu-se ao fato do avestruz ser uma esp?cie ex?tica e pelo n?o conhecimento do
potencial de microrganismos que esta possui. Os animais foram divididos em quatro grupos,
totalizando 80 amostras. A pesquisa da presen?a do agente nos swabs oriundos das aves foi feita
no Lab. de Leveduras. Patog?nicas e Ambientais, Depto de Microbiologia e Imunologia
Veterin?ria, IV, UFRRJ, por processamento dos mesmos, com semeadura em meio de cultivo
?gar Sabouraud dextrose. As confirma??es de isolamento foram realizadas por meio de testes de
macromorfologia, micromorfologia, realiza??o de auxanograma, zimograma e provas
complementares. Do total de amostras processadas da orofaringe, 32,5% obtiveram crescimento
leveduriforme, ?nico ou acompanhado de fungos filamentosos. O crescimento de C. albicans
correspondeu a 65,38% do total de isolados leveduriformes. A maioria dos isolados foi
fortemente positiva para produ??o de protease e aproximadamente metade destas amostras
tamb?m mostraram-se positivas para a produ??o de fosfolipases o que sugere a alta virul?ncia
destas amostras.
Palavras chave: Candida, avestruzes, virul?ncia.
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