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2008 - Pablo Waldeck Goncalves de Souza.pdf: 2011389 bytes, checksum: 2d903c70e0f497dfa879d5f386e128c6 (MD5)
Previous issue date: 2008-03-07 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior === The objective of this paper was determined the presence of Cryptococcus neoformans in
cockroach (Periplaneta americana) collected in public places. The motivation for this
research was based on the fact that this species of cockroach is synanthropic and vehicle of
several pathogenic agents for man and animals. The insect were collected already dead and
also collected alive using different traps, at all, were 150 samples. The research of the
presence of the agents in the insects was made in Laboratory of Pathogenic and
Environmental Yeasts, Department of Microbiology and Immunology Veterinary, Institute
of Veterinary, UFRRJ, by the process of the exoskeleton and the internal content of then,
sowing in cultivation medium containing dopamine. The confirmations of isolation were
made by macromorfology and micromorfology test, realization of auxanographic method
and complementally proofs. Was not obtained isolation of Cryptococcus neoformans of
exoskeleton. Were obtained 5 isolated (3%), starting by the content of the insects that, after
the serogrouping in CGB medium, show them self as belonging to AD serum group. Test
show that 80% of the stumps are big producing of phospholipase and 100% big producing
of protease. We considerate that the cockroaches of the species Periplaneta americana are,potentially, a vector for the studied yeast. === O objetivo deste trabalho foi determinar a presen?a de Cryptococcus neoformans
em baratas (Periplaneta americana) recolhidas de ambientes p?blicos. A motiva??o para
esta pesquisa deve-se ao fato desta esp?cie de barata ser sinantr?pica e veiculadora de
v?rios agentes patog?nicos para o homem e animais. Os insetos foram recolhidos mortos e
tamb?m capturados vivos por meio de duas diferentes armadilhas, totalizando 150
amostras. A pesquisa da presen?a do agente nos insetos foi feita no Laborat?rio de
Leveduras Patog?nicas e Ambientais, Depto de Microbiologia e Imunologia Veterin?ria,
Instituto de Veterin?ria, UFRRJ, por processamento do exoesqueleto e do conte?do interno
dos mesmos, com semeadura em meio de cultivo contendo dopamina. As confirma??es de
isolamento foram realizadas por meio de testes de macromorfologia, micromorfologia,
realiza??o de auxanograma e provas complementares. N?o foi obtido isolamento de
Cryptococcus neoformans de exoesqueleto. Foram obtidos 5 isolamentos (3%) a partir do
conte?do interno dos insetos que, ap?s sorogrupagem em meio CGB revelaram-se como
pertencentes ao sorogrupo AD. Testes evidenciaram que 80% das cepas s?o forte
produtoras de fosfolipase e 100% forte produtoras de protease. Consideramos que as
baratas da esp?cie Periplaneta americana s?o um potencial vetor para a levedura estudada.
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