| Summary: | Made available in DSpace on 2016-04-28T20:15:35Z (GMT). No. of bitstreams: 1
2009 - Nicole Brand Ederli1.pdf: 1323032 bytes, checksum: 394a97d8f4b106b07b28af4a26a7af8d (MD5)
Previous issue date: 2009-02-13 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior === The aim of this work was differentiates infective larvae of Libyostrongylus douglassii, L.
dentatus and Codiostomum struthionis allowing the differential diagnosis of those species
among fecal cultures. For this, 10 adult ostriches were slaughtered, their proventriculum and
caeca ware removed and adult parasites were collected. Females adults of L. douglassii and
L. dentatus were separate in five samples of 100 females each. The samples were macerated
individually and mixed with sterilized feces and culture fecal was performed to obtaining of
infective larvae. Adults parasites of C. struthionis collected in the caeca were washed in
saline solution (NaCl 0,09%) and quantified. All the parasites were fixed in A.F.A. for
optical microscopy and adults of C. struthionis and infective larvae of Libyostrongylus spp.
were fixed in Karnovsky solution for scanning electron microscopy. All proventriculum and
90% of the analyzed caeca were positive to the infection by Libyostrongylus spp. and C.
struthionis, respectively. It is possible to distinguish L. douglassii of L. dentatus and C.
struthionis by the morphology and length of the larvae sheath tail, that in L. douglassii is short
and with acute ending different the other two species that are long and filamentous. For the
differential diagnosis between L. dentatus and C. struthionis is more reliable the use of the
morphology of the ending of the larvae tail that, in L. dentatus presents a knob in the
extremity, this character is not observed in C. struthionis. Like this, this continues being the
best parameter for the differentiation among these two species of gastrointestinals nematodes
of the ostriches. The differentiation of the infective larvae of L. douglassii, L. dentatus and C.
struthionis among fecal cultures will facilitate the diagnosis of this species, by researchers and
breeders, favoring the knowledge of the biology of these nematodes. === O presente trabalho tem como objetivo diferenciar larvas infectantes de Libyostrongylus
douglassii, L. dentatus e Codiostomum struthionis permitindo o diagn?stico diferencial dessas
esp?cies atrav?s de culturas fecais. Para isto, 10 avestruzes adultos foram abatidas, seus
proventr?culos e cecos removidos e coletado parasitas adultos. F?meas adultas L. douglassii e
L. dentatus foram separadas em cinco amostras de 100 f?meas cada. As amostras foram
maceradas individualmente e misturadas com fezes secas esterilizadas e foi realizada cultura
fecal padr?o para obten??o de larvas infectantes. Parasitas adultos de C. struthionis
recolhidos no ceco foram lavados em solu??o fisiol?gica (NaCl 0,09%), quantificados. Todos
os parasitas foram fixados em A.F.A. para microscopia ?ptica e adultos de C. struthionis e
larvas infectantes de Libyostrongylus spp. foram fixados em solu??o Karnovsky para
microscopia eletr?nica de varredura. Todos os proventr?culos e 90% dos cecos analisados
estavam parasitados por Libyostrongylus spp. e C. struthionis, respectivamente. ? poss?vel
distinguir L. douglassii de L. dentatus e C. struthionis pela morfologia e comprimento da
cauda da bainha que em L. douglassii ? curta e com termina??o aguda ao contr?rio das outras
duas esp?cies que s?o longas e filamentosas. Para o diagn?stico diferencial entre L. dentatus
e C. struthionis ? mais confi?vel a utiliza??o da morfologia da termina??o da cauda da larva
que, em L. dentatus apresenta uma protuber?ncia arredondada na extremidade, caractere este
n?o observado em C. struthionis. Assim, este continua sendo o melhor par?metro para a
diferencia??o entre estas duas esp?cies de nemat?ides gastrintestinais das avestruzes. A
diferencia??o das larvas infectantes de L. douglassii, L. dentatus e C. struthionis atrav?s de
culturas fecais ir? facilitar o diagn?stico das esp?cies, por pesquisadores e criadores,
favorecendo o conhecimento da biologia destes nemat?ides.
|
|---|
