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Previous issue date: 2009-10-29 === Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq === This study was carried out to estimate the prececal and total nutrient?s digestibility of forages by total feces collection and mobile bag techniques and evaluate the passage kinetics in the digestive tract of horses using mobile bags. This work was conducted with three assays. The first was carried out to estimate nutrient?s digestibility of coast cross hay (Cynodon dactylon cv. coastcross) with the total feces collection technique. The second assay was carried out to estimate nutrient?s digestibility of lucerne hay (Medicago sativa), coast cross hay, and peanut forage (Arachis pintoi) with the mobile bag technique. The last one was carried out to evaluate the prececal nutrient?s digestibility of the same forages used at second assay with the mobile bag technique in a horse cannulated at cecum. The two first assays were simultaneously, with four horses with average weight of 300kg fed coast cross hay only. Water and mineral salt was offered ad libitum. The nylon bags has 45? of porosity and 6.5x 3.0cm of internal dimension and were fill with 663 mg of forage samples ground at 1mm. These assays had duration of 19 days, 10 days for adaptation and regulation of intake, and the last five days to introduce the bags in the stomach and, bags collections in the feces. Two insertions of bags were made at day with 25 bags, eight bags per forage and one empty bag. The feces and the bags were kept on the floor quickly after the horses crap, the bags were separate and the time noted. The assay of prececal digestibility had duration of 20 days which the first 5 days were to horse adaptation and the last 15 days were to insert and recovery the bags in the cecum. In this assay the bags were similar of those used in the first assay, but with a little washer in the border of the bags to possibility the recovery them in the cecum with a magnet. The magnet was place into the cannula near the ileocaecal junction, and at every 30 minutes beginning one hour after bag?s insertion in the stomach, the magnet was withdraw and the bags was removed until 7 hours later. Values of total digestibility with feces collection and mobile bags were similar with the possibility to use the mobile bag technique as alternative to feces collection. There wasn?t differences between digestibility of nutrients of lucerne and peanut forage with mobile bags technique (P<0.05), nevertheless those were higher than the coast cross hay. The use of mobile bag in cannulated horses allow to estimate the digestibility from different segments of the intestine, show to be a good alternative to study the digestion in horses === Este trabalho teve como objetivo estimar a digestibilidade parcial e total dos nutrientes de forrageiras atrav?s das t?cnicas de coleta total de fezes e sacos de n?ilon m?veis e avaliar a cin?tica de passagem de sacos de n?ilon m?veis no trato digest?rio de equinos. O experimento foi dividido em tr?s ensaios. O primeiro ensaio estimou a digestibilidade dos nutrientes do feno de coastcross (Cynodon dactylon cv. coastcross) atrav?s da coleta total de fezes. O segundo ensaio estimou a digestibilidade dos nutrientes do feno de alfafa (Medicago sativa), do feno de coastcross e do amendoim forrageiro (Arachis pintoi) com a t?cnica de sacos de n?ilon m?veis. O terceiro ensaio foi realizado utilizando as mesmas forrageiras do segundo ensaio, avaliando a digestibilidade pr?-cecal com a t?cnica dos sacos de n?ilon m?veis em equino fistulado no ceco. Os dois ensaios de digest?o iniciais foram realizados simultaneamente, com quatro equinos adultos com peso m?dio de 300kg alimentados com dieta exclusiva de feno de coastcross. A ?gua e o sal mineral foram fornecidos ad libitum. Foram utilizados sacos de n?ilon com porosidade de 45? com dimens?es internas de 6,5x 3cm, e preenchidos com 663mg de amostra das forrageiras mo?das a 1mm. Os dois ensaios iniciais tiveram dura??o de 19 dias, sendo 10 dias de adapta??o e regula??o do consumo da dieta, cinco dias para inser??o g?strica dos sacos, coleta total de fezes e dos sacos de n?ilon nas fezes. Para inser??o g?strica dos sacos de n?ilon, foram feitas duas sondagens ao dia, com 25 sacos, oito com amostras de cada alimento e um em branco. As fezes e os sacos foram coletados do piso das baias imediatamente ap?s a excre??o, os sacos foram separados manualmente e anotado o tempo de tr?nsito. O ensaio de digest?o pr?-cecal teve dura??o de 20 dias sendo os cinco primeiros para adapta??o do animal ao manejo e 15 dias de sondagens e recupera??o dos sacos. Neste ensaio foram utilizados sacos semelhantes ao ensaio anterior, por?m em uma das extremidades dos sacos foi afixada uma arruela met?lica para permitir que o saco fosse recuperado no ceco com aux?lio de um im?. Este im? foi posicionado pr?ximo a jun??o ?leo-cecal e, a intervalos de 30 minutos, iniciando uma hora ap?s a inser??o dos sacos no estomago e, o im? foi retirado da c?nula e os sacos aderidos foram coletados at? 7 horas ap?s a inser??o g?strica. Os valores de digestibilidade total atrav?s da coleta de fezes e dos sacos m?veis foram semelhantes confirmando a possibilidade de utiliza??o dos sacos m?veis como alternativa a coleta de fezes. N?o houve diferen?a entre os coeficientes de digestibilidade do feno de alfafa e do amendoim forrageiro com os de sacos de n?ilon m?veis (P<0,05), no entanto estes foram maiores que o do feno de coastcross. O uso da t?cnica de sacos de n?ilon m?veis em animais fistulados permitiu estimar a digestibilidade pr?-cecal e p?s-ileal em equinos, mostrando ser uma alternativa vi?vel no estudo dos processos digestivos desta esp?cie.
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