Avalia??o hematol?gica, bioqu?mica, cl?nica e diagn?stico molecular de agentes micoplasmas hemotr?ficos em c?es dom?sticos oriundos de abrigos da regi?o metropolitana do Rio de Janeiro

• Main Author: SILVA, Aline Tonussi da
• Other Authors: Baldani, Cristiane Divan Baldani
• Format: Others
• Language: Portuguese
• Published: Universidade Federal Rural do Rio de Janeiro 2017
• Subjects: Domestic dogs; Hemoplasmas; Shelters; C?es dom?sticos; Abrigos.; Patologia Animal
• Online Access: https://tede.ufrrj.br/jspui/handle/jspui/1430

Submitted by Jorge Silva ([e-mail]) on 2017-02-14T18:58:03Z No. of bitstreams: 1 2016 - Aline Tonussi da Silva.pdf: 2072458 bytes, checksum: 143dab8a0068b4bd1a643fe48f82873c (MD5) === Made available in DSpace on 2017-02-14T18:58:03Z (GMT). No. of bitstreams: 1 2016 - Aline Tonussi da Silva.pdf: 2072458 bytes, checksum: 143dab8a0068b4bd1a643fe48f82873c (MD5) Previous issue date: 2016-02-29 === CAPES === The hemotropic hemoparasites mycoplasmas are gram-negative bacteria, which must attach to the wall of erythrocytes, causing deformation in the membrane of the infected erythrocytes. The goal of this study was to detect molecularly hemoplasmas infection in sheltered dogs in Metropolitan region of Rio de Janeiro, linking it to hematological, biochemical and clinical data changes. Of 222 dogs sampled, 20,27% (45/222) were positive by the Polymerase Chain Reaction Real Time (qPCR) for Mycoplasma spp.. The frequency of hemoplasma species infection by PCR using species-specific oligonucleotides, was 13,06% (29/222), 0% (0/222) and 2,25% (5/222) respectively for M. haemocanis (Mhc), ?Candidatus M. turicensis? (Cmt) and ?Candidatus Mycoplasma haematoparvum / haemominutum? (Cmhp / m). There for e M. haemocanis presented the highest incidence in the dogs population studied. Coinfections were identified between Cmhp/m and Mhc 1,35% (3/222). Regarding hematological alterations associated with infection by Mycoplasma spp., hyperproteinemia on plasma was detected (p = 0,0010). It was not observed an association between hemoplasmas infection and the occurrence of anemia and leukocyte changes. Changes in serum biochemistry showed a significant difference in serum protein (p = 0,0291), globulin (p = 0,0253), creatinine (p = 0,0279) and alkaline phosphatase (p = 0,0242). Additionally the azotemia was statistically (p = 0,0023) associated with the infection. The results obtained in the evaluation of Mhc positive dogs demonstrated no significant difference in plasma total protein (p = 0,0012) and hyperproteinaemia was associated with infection (p = 0,0238). There were significant differences in alkaline phosphatase parameter (p = 0,0173) and urea (p = 0,0401), with azotemia is associated shall infection (p = 0,0061). The results observed in the evaluation of positive dogs for (Cmhp / m) show that ALT (alanine aminotransferase) was associated with infection (p = 0,0301). As for factors associated with infection by Mycoplasma spp., only gender (p = 0,0062) and age (p = 0,0041) were associated with infec??o.N?o are statistically associated with the historical agent of fights, access to street, castration, history fleas, ticks history, presence of fleas and ticks. The frequency of the main findings of anamanse and clinical examination of the dogs positive for Mycoplasma spp . were lymphadenopathy (37,8% ) , skin lesions compatible with scabies ( 28,9%) , TPC > 2 ( 31,1% ) , oral affections ( 15,5%) of hist?rico ehrlichiosis ( 13,3%) . Consequently, knowledge of the disease and the factors related to their occurrence are necessary for the adoption of preventive measures aimed at animal health. === Os hemoparasitos micoplasmas hemotr?ficos s?o bact?rias gram-negativas, que se fixam obrigatoriamente na superf?cie dos eritr?citos, levando a deforma??o desta. O objetivo do presente estudo foi detectar molecularmente a infec??o por hemoplasmas em c?es de abrigos da Regi?o Metropolitana do Rio de Janeiro, associando-a aos dados hematol?gicos, bioqu?micos e altera??es cl?nicas. Dos 222 c?es amostrados, 20,27% (45/222) foram positivos pela Rea??o em Cadeia da Polimerase em Tempo Real (qPCR) para Mycoplasma spp.. A frequ?ncia de infec??o das esp?cies de hemoplasma pela PCR, utilizando oligonucleot?deos esp?cie-espec?ficos, foi 13,06% (29/222), 0% (0/222) e 2,25% (5/222), respectivamente para M. haemocanis (Mhc), ?Candidatus M. turicensis (CMt) e ?Candidatus Mycoplasma haematoparvum/ haemominutum? (CMhp/m). M. haemocanis constitui, portanto, a esp?cie de maior ocorr?ncia na popula??o de c?es estudada. Foram identificadas coinfec??es entre CMhp/m e Mhc em 1,35% (3/222) dos c?es. No tocante ?s altera??es hematol?gicas associadas ? infec??o por Mycoplasma spp., constatou-se hiperproteinemia plasm?tica refratom?trica (p=0,0010). N?o foi observada associa??o entre a infec??o por hemoplasmas e a ocorr?ncia de anemia ou altera??es leucocit?rias. Nas altera??es da bioqu?mica sangu?nea, evidenciou-se diferen?a significativa na prote?na s?rica (p=0,0291), globulina (p=0,0253), creatinina (p=0,0279), fosfatase alcalina (p=0,0242). Adicionalmente, a azotemia foi estatisticamente (p=0,0023) associada ? infec??o. Os resultados observados na avalia??o de c?es positivos para Mhc demonstram haver diferen?a significativa na prote?na plasm?tica total (p=0,0012) e hiperproteinemia foi associada ? infec??o (p=0,0238). Houve diferen?a significativa no par?metro fosfatase alcalina (p=0,0173) e ur?ia (p=0,0401), com a azotemia sendo associada ? infec??o (p=0,0061). Os resultados observados na avalia??o de c?es positivos para CMhp/hm demonstram que a ALT (alanina aminotransferase) foi associada ? infec??o (p=0,0301). Quanto aos fatores associados ? infec??o por Mycoplasma spp., apenas g?nero (p=0,0062) e idade (p=0,0041) foram associadas ? infec??o. N?o apresentaram associa??o estat?stica com o agente hist?rico de brigas, acesso a rua, castra??o, hist?rico de pulgas, hist?rico de carrapatos, presen?a de pulgas e presen?a de carrapatos. A frequ?ncia dos principais achados de anamanse e exame cl?nico dos c?es positivos para Mycoplasma spp. foram a linfadenopatia (37,8%), les?es de pele compat?veis com sarna (28,9%), TPC >2 (31,1%), afec??es bucais (15,5%) e hist?rico de erlichiose (13,3%). Consequentemente, o conhecimento da doen?a bem como dos fatores relacionados ? sua ocorr?ncia s?o necess?rios para a ado??o de medidas preventivas visando ? sa?de animal.