Co-inocula??o de riz?bio e bact?rias promotoras de crescimento vegetal em feijoeiro comum

• Main Author: BASTOS, Rennan do Amaral
• Other Authors: Ara?jo, Adelson Paulo de
• Format: Others
• Language: Portuguese
• Published: Universidade Federal Rural do Rio de Janeiro 2017
• Subjects: Co-inoculation; Growth; Phaseolus vulgaris; Co-inocula??o; Crescimento; Agronomia - Ci?ncia do Solo
• Online Access: https://tede.ufrrj.br/jspui/handle/jspui/1412

Submitted by Jorge Silva ([e-mail]) on 2017-02-08T16:51:22Z No. of bitstreams: 1 2016 - Rennan do Amaral Bastos.pdf: 2824478 bytes, checksum: 5a974a7444eaef272210a147add547a0 (MD5) === Made available in DSpace on 2017-02-08T16:51:22Z (GMT). No. of bitstreams: 1 2016 - Rennan do Amaral Bastos.pdf: 2824478 bytes, checksum: 5a974a7444eaef272210a147add547a0 (MD5) Previous issue date: 2016-02-04 === The objective of this work was to evaluate the production of biomass and grains in common bean (Phaseolus vulgaris) in co-inoculation with rhizobia and plant growth promoting bacteria. The work was divided into three chapters. The first evaluated the compatibility between rhizobia and plant growth promoting bacteria and their effects on plant growth. In Petri dishes assay, Azospirillum amazonense and A. brasilense were co-inoculated with Rhizobium tropici at three application levels, and no antagonism was detected. The experiment in Leonard jars had a 5x3+3 factorial with eight replications, consisting of five bacteria (Bradyrhizobium diazoefficiens, B. elkanii, A. amazonense, Azospirillum brasilense and Escherichia coli) and three application levels (104, 106 and 108 CFU mL-1), all co-inoculated with R. tropici plus three additional treatments (control, nitrogen, and inoculated with R. tropici). Co-inoculation increased nodulation, N concentration and content in the shoot. The experiment in pots with soil had 3x2x2+4 factorial with four replications, consisting of three growth promoters (B. diazoefficiens, B. elkanii and A. brasilense), two application levels (104 and 106 CFU mL-1), two N sources (R. tropici and mineral N), and four additional control treatments (absolute control, nitrogen, inoculated with rhizobia and co-inoculated with AZOTOTAL?). Inoculation with A. brasilense associated with N fertilization increased the production of leaves and shoots. The second chapter aimed to evaluate the growth and production of common bean under co-inoculation. An experiment in pots with soil in a factorial 2x3+4 with 8 repetitions were performed by combining the inoculation of R. tropici with A. brasilense or B. diazoefficiens in three application levels (104, 106 and 108 CFU mL-1) plus the control treatments (absolute, nitrogen, inoculated with rhizobia and co-inoculated with AZOTOTAL?). Four replications were harvested at 45 days after sowing, and four at grain maturity. Co-inoculated plants showed shoot mass, number of pods, shoot N content, grain yield, number of seeds and pods per plant higher than the control inoculated with rhizobia. The leaf chlorophyll Falker index was similar in co-inoculated treatments and under mineral N fertilization, but lower than the exclusive inoculation R. tropici. In the third chapter it was evaluated the growth and production of common bean under different strategies of mineral N fertilization associated with co-inoculation with R. tropici and A. brasilense. The experiment had seven treatments with four replications: control; inoculation with R. tropici without N application; control with mineral N; co-inoculation with R. tropici and A. brasilense without N fertilization or application of 20 kg ha-1 of N at sowing; inoculation with R. tropici and 20 kg ha-1 of N at sowing; inoculation with A. brasilense and 20 kg ha-1 of N at sowing. Plants were harvested at 45 days after sowing and at grain maturity. Co-inoculation combined with the N fertilization resulted in higher mass values of leaves, stem, shoot, dry mass of grains, number of grains per plant and accumulation of N in pods and grains. === O objetivo do trabalho foi avaliar a produ??o de biomassa e de gr?os em feijoeiro comum (Phaseolus vulgaris) sob co-inocula??o de riz?bio e bact?rias promotoras de crescimento vegetal. O trabalho foi dividido em tr?s cap?tulos. O primeiro teve como objetivo avaliar a compatibilidade entre riz?bio e bact?rias promotoras de crescimento e seus efeitos no crescimento das plantas. Em ensaio em placas de Petri, Azospirillum amazonense e A. brasilense foram co-inoculados com Rhizobium tropici em tr?s doses de aplica??o, verificando-se aus?ncia de antagonismo. O experimento em vasos de Leonard teve esquema fatorial 5x3+3 com oito repeti??es, composto por cinco bact?rias (Bradyrhizobium diazoefficiens, B. elkanii, Azospirillum amazonense, A. brasilense e Escherichia coli) e tr?s doses de aplica??o (104, 106 e 108 UFC mL-1), todas co-inoculadas com R. tropici, e tr?s tratamentos adicionais (testemunhas absoluta, nitrogenada, e inoculada com R. tropici). A co-inocula??o aumentou a nodula??o, teor e conte?do de N na parte a?rea. O experimento em vasos com solo teve esquema fatorial 3x2x2+4 com quatro repeti??es, composto por tr?s promotores de crescimento (B. diazoefficiens, B. elkanii e A. brasilense), duas doses (104 e 106 UFC mL-1), duas fontes de N (R. tropici e N mineral), e quatro tratamentos adicionais (testemunhas absoluta, nitrogenada, inoculada com riz?bio e co-inoculada com AZOTOTAL?). A inocula??o com A. brasilense associada ? aduba??o nitrogenada aumentou a produ??o de folhas e de parte a?rea. O segundo cap?tulo teve como objetivo avaliar o crescimento e produ??o do feijoeiro sob co-inocula??o. Foi realizado um experimento em vasos com solo em esquema fatorial 2x3+4 com oito repeti??es, combinando a inocula??o de R. tropici com A. brasilense ou B. diazoefficiens e tr?s doses de aplica??o (104, 106 e 108 UFC mL-1), acrescidos das testemunhas absoluta, nitrogenada, inoculada com riz?bio e co-inoculada com AZOTOTAL?. As plantas foram coletadas aos 45 dias ap?s plantio e na matura??o dos gr?os. As plantas co-inoculadas apresentaram massa de parte a?rea, n?mero de vagens, conte?do de N na parte a?rea, massa de gr?os, n?mero de gr?os e de vagens por planta, superiores ? testemunha inoculada com riz?bio. O ?ndice de clorofila Falker foi similar nos tratamentos co-inoculados e sob aduba??o nitrogenada, mas inferior ao da inocula??o com R. tropici. No terceiro cap?tulo avaliou-se o crescimento e produ??o do feijoeiro sob diferentes estrat?gias de aduba??o nitrogenada associada ? co-inocula??o. O experimento consisitu de sete tratamentos com oito repeti??es: testemunha absoluta; inocula??o com R. tropici sem aplica??o de N; testemunha com N mineral; co-inocula??o com R. tropici e A. brasilense sem N ou com 20 kg ha-1 de N em semeadura; inocula??o com R. tropici e 20 kg ha-1 de N em semeadura; inocula??o com A. brasilense e 20 kg ha-1 de N em semeadura. As plantas foram coletadas aos 45 dias e na matura??o. A co-inocula??o combinada com a aduba??o nitrogenada resultou em maiores valores de massa de folhas, caule, parte a?rea, massa seca de gr?os, n?mero de gr?os por planta e ac?mulo de N nas vagens e gr?os.