| Summary: | === The aim of the present study is to evaluate in vitro lethal sensibilization of periodontopathogenic bacteria: Fusobacterium nucleatum (Fn), Actinobacillus actinomycetemcomitans (Aa) and Prevotella intermedia (Pi) by using toluidine blue (0.01% w/v) as photo sensitizer and comparing the irradiation of identical solutions of bacteria with photo sensitizer by three light sources: two lasers systems of different companies (660 nm) and one LED equipment (light emitting diode, 630 nm). For each species, they were established a control group and
different test groups, among them: Group 6 (Fn, Aa and Pi) bacteria solution irradiated by laser 1; Group 7 (Fn, Aa and Pi) bacteria solution irradiated by laser 2 and Group 8 (Fn, Aa and Pi) bacteria solution irradiated by LED. Irradiation protocol followed the literature recommendations: 5 minutes of preirradiation time and 3 minutes of irradiation. Results reveal that the bacteria reduction for the irradiated groups (6, 7 and 8) in comparison with control group is statistically significant (p<0.05). Actinobacillus actinomycetemcomitans
presented important level of reduction (99.8% for Group 6Aa, 81.9% for Group 7Aa and 99.8% for Group 8Aa) as well as Fusobacterium nucleatum (93.7% for Group 6Fn, 84% for Group 7Fn and 99.8% for Group 8Fn). Prevotella intermedia presented low level of reduction (26.3% for Group 6Pi, 38.6% for Group 7Pi and 49.5% for Group 8Pi). Toluidine blue at concentration of 0.01% w/v is an effective photosensitizer for Actinobacillus actinomycetemcomitans and Fusobacterium nucleatum when irradiated with the light wavelength in the range of 630 to 660 nm using laser or LED light sources. === O objetivo deste estudo foi avaliar e comparar a sensibilizacao letal in vitro das bacterias periodontopatogenicas (Fusobacterium nucleatum, Actinobacillus Actinomycetemcomitans e Prevotella intermedia), atraves da utilizacao de lasers e LED (Light Emitting Diode) no processo de reducao bacteriana, utilizando como fotossensibilizador azul de toluidina (TBO) a 0,01%. Os resultados estatisticos obtidos pelo Teste t-student demonstraram que os grupos de terapia fotodinamica (PDT) (grupos 6, 7, 8) sao diferentes do grupo controle para p=0,05 e os valores percentuais de reducao bacteriana dos grupos PDT foram de 99,8% no grupo 6, 81,9% no grupo 7 e 99,8% no grupo 8 para o Actinobacillus actinomycetemcomitans, de 93,7% no grupo 6, 84% para o grupo 7 e 99,8% no grupo 8 para o Fusobacterium nucleatum e 26,3% no grupo 6, 38,6% no grupo 7 e 49,5% no grupo 8 para a Prevotella intermedia,
demonstrando assim, que o corante azul de toluidina 0,01% associado aos lasers e ao LED apresenta um excelente potencial para a utilizacao em PDT, na sensibilizacao letal dessas bacterias nos parametros utilizados no experimento (¢t=3minutos).
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