Caracteriza??o morfol?gica e molecular de gen?tipos de batata-doce

• Main Author: Andrade, Elis?ngela Knoblauch Viega de
• Other Authors: Andrade J?nior, Valter Carvalho de
• Language: Portuguese
• Published: UFVJM 2016
• Subjects: Ipomoea batatas (L.); Marcadores morfol?gicos; Microssat?lites; Diversidade gen?tica; Convolvulaceae
• Online Access: http://acervo.ufvjm.edu.br/jspui/handle/1/800

Submitted by Rodrigo Martins Cruz ([e-mail]) on 2016-01-05T12:38:46Z No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) === Approved for entry into archive by Rodrigo Martins Cruz ([e-mail]) on 2016-01-05T17:44:56Z (GMT) No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) === Made available in DSpace on 2016-01-05T17:44:56Z (GMT). No. of bitstreams: 2 elisangela_knoblauch_viega_andrade.pdf: 1007346 bytes, checksum: 82e99aa23997633aaed7026a8489fe65 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2014 === Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) === Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) === Objetivou-se realizar a caracteriza??o morfol?gica e molecular de gen?tipos de batata-doce do banco de germoplasma da Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM). Para a caracteriza??o morfol?gica, o experimento foi conduzido no Setor de Olericultura da UFVJM sendo avaliados 60 gen?tipos de batata-doce, utilizando 24 descritores morfol?gicos, tanto da parte a?rea quanto da parte radicular. As avalia??es da parte a?rea foram realizadas 60 e 90 dias ap?s o plantio, e as avalia??es da parte radicular foram realizadas ap?s a colheita aos 150 dias ap?s o plantio. Para a caracteriza??o molecular foram utilizadas amostras foliares de 60 acessos oriundos do banco de germoplasma-UFVJM. A extra??o do DNA foi realizada no Laborat?rio de Gen?tica e Biotecnologia Florestal (UFVJM), utilizando o m?todo Fenol-Clorof?rmio. Para a amplifica??o do DNA, foram utilizados onze primers microssat?lites espec?ficos para a batata-doce. A diversidade morfol?gica e molecular foi obtida por meio da gera??o de matrizes de dissimilaridade baseando-se no coeficiente de coincid?ncia simples e ?ndices de Jaccard, respectivamente. Para o estudo da diverg?ncia recorreu-se ao dendrograma ilustrativo obtido pelo m?todo UPGMA e ao agrupamento de Tocher. Para as matrizes de dissimilaridade obtida com os dados morfol?gicos e moleculares foram estimadas dist?ncias gen?ticas variando de 0,08 a 0,92 com m?dia 0,52 e 0,14 a 1, com m?dia 0,76, respectivamente. Observou-se pelos dendrogramas de dissimilaridade e pelo agrupamento de Tocher, que os acessos de batata-doce do banco de germoplasma da UFVJM apresentaram diversidade gen?tica e fenot?pica, sendo promissores em programas de melhoramento desta cultura. === Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. === ABSTRACT The objective was to make the morphological and molecular characterization of sweet potato genotypes of the germplasm bank of the Federal University of the Jequitinhonha and Mucuri Vales (UFVJM). For morphological characterization, the experiment was conducted in the Horticulture Sector of UFVJM being evaluated 60 sweet potato genotypes, using 24 morphological descriptors of both the shoots as the root part. The shoot evaluations were performed 60 and 90 days after planting, and ratings of roots were done after harvest at 150 days after planting. The molecular characterization leaf samples from 60 hits coming from the germplasm bank-UFVJM were used. DNA extraction was performed in Forest Genetics and Biotechnology Laboratory (UFVJM) using the phenol-chloroform method. For amplification of DNA eleven microsatellite primers specific for the sweet potato were used. The morphological diversity and Molecular was obtained by generating dissimilarity matrices based on the simple matching coefficient of Jaccard indices, respectively. To study the divergence appealed to the illustrative dendrogram by UPGMA and Tocher grouping. For dissimilarity matrices obtained with morphological and molecular data were estimated genetic distances ranging from 0,08 to 0,92 with a mean 0,52 and 0,14 to 1, with mean 0,76, respectively. It was observed by dendrograms of dissimilarity and the Tocher group, the sweet potato accessions of germplasm bank of UFVJM showed genetic and phenotypic diversity, and promising in breeding programs of this culture.