Sele??o de gen?tipos e an?lise da express?o g?nica diferencial induzida por Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) em Eucalyptus spp.

• Main Author: Ferreira, Marcele dos Santos
• Other Authors: Barbosa, Leonardo Rodrigues
• Language: Portuguese
• Published: UFVJM 2015
• Subjects: Percevejo bronzeado; Biblioteca de cDNA; Gen?mica funcional; mRNA
• Online Access: http://acervo.ufvjm.edu.br:8080/jspui/handle/1/351

Submitted by Rodrigo Martins Cruz ([e-mail]) on 2015-01-07T10:54:29Z No. of bitstreams: 2 marcele_santos_ferreira.pdf: 1547534 bytes, checksum: a0861513c3025ada252d9e4fa8f0c494 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) === Approved for entry into archive by Rodrigo Martins Cruz ([e-mail]) on 2015-01-07T10:55:14Z (GMT) No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) marcele_santos_ferreira.pdf: 1547534 bytes, checksum: a0861513c3025ada252d9e4fa8f0c494 (MD5) === Approved for entry into archive by Rodrigo Martins Cruz ([e-mail]) on 2015-01-07T10:55:10Z (GMT) No. of bitstreams: 2 marcele_santos_ferreira.pdf: 1547534 bytes, checksum: a0861513c3025ada252d9e4fa8f0c494 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) === Approved for entry into archive by Rodrigo Martins Cruz ([e-mail]) on 2015-01-07T10:55:34Z (GMT) No. of bitstreams: 2 marcele_santos_ferreira.pdf: 1547534 bytes, checksum: a0861513c3025ada252d9e4fa8f0c494 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) === Made available in DSpace on 2015-01-07T10:55:34Z (GMT). No. of bitstreams: 2 marcele_santos_ferreira.pdf: 1547534 bytes, checksum: a0861513c3025ada252d9e4fa8f0c494 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2013 === Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) === Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) === Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) === O g?nero Eucalyptus engloba centenas de esp?cies conhecidas e de elevado interesse comercial. Este g?nero florestal ? altamente respons?vel pelo abastecimento da cadeia produtiva da madeira no Brasil. Diversos aspectos podem afetar negativamente a produtividade dos plantios, como, por exemplo, a ocorr?ncia de pragas e doen?as. O percevejo bronzeado Carpintero e Dellap?, 2006 (Hemiptera: Thaumastocoridae) ? uma praga ex?tica, nova no Brasil. Uma estrat?gia para diminuir os efeitos negativos dos insetos-praga ? selecionar materiais gen?ticos resistentes. O isolamento e a identifica??o de genes envolvidos no processo de resist?ncia podem ser usados na obten??o de indiv?duos com caracter?sticas desej?veis. O presente trabalho teve por objetivos a avalia??o de materiais gen?ticos de Eucalyptus spp. quanto ? inj?ria ocasionada pelo percevejo bronzeado e a constru??o, a partir de materiais contrastantes, de bibliotecas subtrativas de cDNA. A escolha dos gen?tipos baseou-se em metodologias distintas, conduzidas em laborat?rio, sala climatizada e em casa de vegeta??o. Foram estudados 27 clones h?bridos. Os gen?tipos C03 e C17 foram escolhidos e, juntamente com mudas de E. camaldulensis, submetidos ao ataque de T. peregrinus. A partir das amostras de RNA mensageiro foi poss?vel construir duas bibliotecas subtrativas: uma com genes diferencialmente expressos entre o clone C03 e o E. camaldulensis e outra a partir do clone C17 e o E. camaldulensis. === Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. === ABSTRACT The genus Eucalyptus encompasses hundreds of known species with high commercial interest. This forest genus is largely responsible for the supply of the wood production chain in Brazil. Several aspects can negatively affect the plantation productivity, for example the occurrence of pests and diseases. The bronze bug Carpintero e Dellap?, 2006 (Hemiptera:Thaumastocoridae) is a new exotic pest in Brazil. A strategy to reduce the negative effects of the insect pests is to select genetic material with resistance. The isolation and identification of resistance genes can be used in order to obtain individuals with desirable characteristics. The present study aimed to evaluate genetic materials of Eucalyptus spp. under the attack of Thaumastocoris peregrinus and make cDNA libraries from contrasting materials. The choice of genotypes relied on different methodologies conducted in laboratory, climate-controlled room and greenhouse. There were studied 27 hybrid clones. The genotypes C03 and C17, were selected along with E. camaldulensis seedlings and subjected to the attack of T. peregrinus. Two genomic subtractive libraries were made from the total RNA samples, one containing the differentially expressed genes between C03 and E. camaldulensis, and another from C17 and E. camaldulensis.