Expression, Purification, and Characterization of the SIAA M79A Protein

Some pathogenic bacteria derive significant amounts of iron heme from their hosts. In this study we investigated SiaA, a heme binding protein from Streptococcus pyogenes. The wildtype methionine79 putative axial ligand was mutated to alanine. SiaA M79A was expressed in E. coli in three production...

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Bibliographic Details
Main Author: Basden, Brian
Format: Others
Published: Digital Archive @ GSU 2007
Subjects:
Online Access:http://digitalarchive.gsu.edu/chemistry_hontheses/1
http://digitalarchive.gsu.edu/cgi/viewcontent.cgi?article=1000&context=chemistry_hontheses