A Hierarchical Graph for Nucleotide Binding Domain 2

• Main Author: Kakraba, Samuel
• Format: Others
• Language: English
• Published: Digital Commons @ East Tennessee State University 2015
• Subjects: Cystic fibrosis; Mutation; Graph-theoretic Models; NBD2; Molecular Descriptors; Nested Graph.; Bioinformatics; Discrete Mathematics and Combinatorics; Epidemiology; Other Applied Mathematics
• Online Access: https://dc.etsu.edu/etd/2517; https://dc.etsu.edu/cgi/viewcontent.cgi?article=3895&context=etd

One of the most prevalent inherited diseases is cystic fibrosis. This disease is caused by a mutation in a membrane protein, the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is known to function as a chloride channel that regulates the viscosity of mucus that lines the ducts of a number of organs. Generally, most of the prevalent mutations of CFTR are located in one of two nucleotide binding domains, namely, the nucleotide binding domain 1 (NBD1). However, some mutations in nucleotide binding domain 2 (NBD2) can equally cause cystic fibrosis. In this work, a hierarchical graph is built for NBD2. Using this model for NBD2, we examine the consequence of single point mutations on NBD2. We collate the wildtype structure with eight of the most prevalent mutations and observe how the NBD2 is affected by each of these mutations.