Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in <em>Sarcophaga crassipalpis</em>.

• Main Author: Ball, Elizabeth Nicole
• Format: Others
• Published: Digital Commons @ East Tennessee State University 2002
• Subjects: Diapause; Ecdysone Receptor; Sarcophaga crassipalpis; Biology; Life Sciences
• Online Access: https://dc.etsu.edu/etd/663; https://dc.etsu.edu/cgi/viewcontent.cgi?article=1820&amp;context=etd

The purpose of this study was to begin a study characterizing the diapause state in Sarcophaga crassipalpis. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing S. crassipalpis were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in S. crassipalpis is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from S. crassipalpis by RT-PCR. This partial EcR was found in all developmental stages tested.