Aufbau eines Screeningverfahrens zur Durchmusterung von
Variantenbibliotheken der T7-RNA-Polymerase hinsichtlich des
Einbaus 2’-Methoxy-modifizierter Nucleotide

Aufbau eines Screeningverfahrens zur Durchmusterung von Variantenbibliotheken der T7-RNA-Polymerase hinsichtlich des Einbaus 2’-Methoxy-modifizierter Nucleotide

Thema dieser Arbeit ist die evolutive Optimierung der T7-RNA-Polymerase. Zur Stabilisierung technischer oder therapeutischer RNA-Moleküle gegenüber RNAsen wäre es wünschenswert eine RNA-Polymerase zu generieren, welche RNA vollständig aus 2’- modifizierten Nucleotiden synthetisieren kann. Zu diesem...

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Bibliographic Details
Main Author:	Nöbel, Nico
Other Authors:	Universität Leipzig, Fakultät für Biowissenschaften, Pharmazie und Psychologie
Format:	Doctoral Thesis
Language:	deu
Published:	Universitätsbibliothek Leipzig 2011
Subjects:	T7 RNA Polymerase Screening evolutive Optimierung 2\'-modifizierte Nucleotide assay direkted evolution modified nucleotides ddc:570
Online Access:	http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-78148 http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-78148 http://www.qucosa.de/fileadmin/data/qucosa/documents/7814/Dis%20final%20Datum.pdf

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