Novel concepts for lipid identification from shotgun mass spectra using a customized query language

• Main Author: Herzog, Ronny
• Other Authors: Technische Universität Dresden, Fakultät Informatik
• Format: Doctoral Thesis
• Language: English
• Published: Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden 2012
• Subjects: Bioinformatik; Formale Sprachen; Massenspektrometry; Lipide; Lipidomics; Lipidanalyse; computational biology; formal languages; mass spectrometry; lipids; lipidomics; lipid analysis; ddc:004; rvk:WC 7700
• Online Access: http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-90497; http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-90497; http://www.qucosa.de/fileadmin/data/qucosa/documents/9049/thesis_v_10_07_12-with_tif_images.pdf

Lipids are the main component of semipermeable cell membranes and linked to several important physiological processes. Shotgun lipidomics relies on the direct infusion of total lipid extracts from cells, tissues or organisms into the mass spectrometer and is a powerful tool to elucidate their molecular composition. Despite the technical advances in modern mass spectrometry the currently available software underperforms in several aspects of the lipidomics pipeline. This thesis addresses these issues by presenting a new concept for lipid identification using a customized query language for mass spectra in combination with efficient spectra alignment algorithms which are implemented in the open source kit “LipidXplorer”.