An Evanescent Perspective on Cells
<p>We have optically sectioned living cells to a maximum depth of ~250 nm using a Variable Angle-Total Internal Reflection Fluorescence Microscope (VA-TIRFM). This yields 3D images of cell membranes and nearby organelles similar to that gained by confocal microscopes but with at least an order...
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https://thesis.library.caltech.edu/6027/2/02_Chapter_2_LWade.pdf
https://thesis.library.caltech.edu/6027/3/03_Chapter_3_LWade.pdf
https://thesis.library.caltech.edu/6027/4/04_Chapter_4_LWade.pdf
https://thesis.library.caltech.edu/6027/5/05_Appendix_A_LWade.pdf
https://thesis.library.caltech.edu/6027/6/06_Appendix_B_LWade.pdf
https://thesis.library.caltech.edu/6027/7/07_Appendix_C_LWade.pdf
https://thesis.library.caltech.edu/6027/8/08_Appendix_D_LWade.pdf
https://thesis.library.caltech.edu/6027/9/09_Appendix_E_LWade.pdf
https://thesis.library.caltech.edu/6027/10/10_Appendix_F_LWade.pdf
https://thesis.library.caltech.edu/6027/11/11_Appendix_G_LWade.pdf
https://thesis.library.caltech.edu/6027/12/12_Appendix_H_LWade.pdf
Wade, Lawrence A. (2011) An Evanescent Perspective on Cells. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/AGAJ-PE93. https://resolver.caltech.edu/CaltechTHESIS:09102010-145946974 <https://resolver.caltech.edu/CaltechTHESIS:09102010-145946974>