Studies in enzyme crystallography

This thesis presents X-ray crystallographic research on chymotrypsin and lysozyme. Seven crystal types of chymotrypsin were identified -- five for the first time. The seven crystal types appear to be different packings of two conformations of the same molecule. One crystal type of lysozyme was st...

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Main Author: Brueckner, David Armstrong
Format: Others
Published: 1966
Online Access:https://thesis.library.caltech.edu/5776/1/Brueckner_da_1966.pdf
Brueckner, David Armstrong (1966) Studies in enzyme crystallography. Master's thesis, California Institute of Technology. doi:10.7907/W06N-8494. https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235 <https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235>
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spelling ndltd-CALTECH-oai-thesis.library.caltech.edu-57762019-12-22T03:09:14Z Studies in enzyme crystallography Brueckner, David Armstrong This thesis presents X-ray crystallographic research on chymotrypsin and lysozyme. Seven crystal types of chymotrypsin were identified -- five for the first time. The seven crystal types appear to be different packings of two conformations of the same molecule. One crystal type of lysozyme was studied. From Patterson projections of crystals diffused with PtI_6^= ions, the x, y, and z coordinates for these ions were determined. After one least squares refinement of these coordinates, the agreement factor between calculated and observed contributions of the PtI_6^= ions was 0.34 for 108 hkO and hOl reflections. Using these coordinates, an hkO and an hOl Fourier projection were prepared. X and y coordinates for the heavy atoms in lysozyme crystals diffused with UO_2^(++) ions, WO_4^= ions, HgI_4^= ions, HgCl_2, ThCl_4, and p-ClHgфSO_3H were determined from Patterson and Fourier projections. 1966 Thesis NonPeerReviewed application/pdf https://thesis.library.caltech.edu/5776/1/Brueckner_da_1966.pdf https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235 Brueckner, David Armstrong (1966) Studies in enzyme crystallography. Master's thesis, California Institute of Technology. doi:10.7907/W06N-8494. https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235 <https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235> https://thesis.library.caltech.edu/5776/
collection NDLTD
format Others
sources NDLTD
description This thesis presents X-ray crystallographic research on chymotrypsin and lysozyme. Seven crystal types of chymotrypsin were identified -- five for the first time. The seven crystal types appear to be different packings of two conformations of the same molecule. One crystal type of lysozyme was studied. From Patterson projections of crystals diffused with PtI_6^= ions, the x, y, and z coordinates for these ions were determined. After one least squares refinement of these coordinates, the agreement factor between calculated and observed contributions of the PtI_6^= ions was 0.34 for 108 hkO and hOl reflections. Using these coordinates, an hkO and an hOl Fourier projection were prepared. X and y coordinates for the heavy atoms in lysozyme crystals diffused with UO_2^(++) ions, WO_4^= ions, HgI_4^= ions, HgCl_2, ThCl_4, and p-ClHgфSO_3H were determined from Patterson and Fourier projections.
author Brueckner, David Armstrong
spellingShingle Brueckner, David Armstrong
Studies in enzyme crystallography
author_facet Brueckner, David Armstrong
author_sort Brueckner, David Armstrong
title Studies in enzyme crystallography
title_short Studies in enzyme crystallography
title_full Studies in enzyme crystallography
title_fullStr Studies in enzyme crystallography
title_full_unstemmed Studies in enzyme crystallography
title_sort studies in enzyme crystallography
publishDate 1966
url https://thesis.library.caltech.edu/5776/1/Brueckner_da_1966.pdf
Brueckner, David Armstrong (1966) Studies in enzyme crystallography. Master's thesis, California Institute of Technology. doi:10.7907/W06N-8494. https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235 <https://resolver.caltech.edu/CaltechTHESIS:05062010-111323235>
work_keys_str_mv AT bruecknerdavidarmstrong studiesinenzymecrystallography
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